Abstract | BACKGROUND: OBJECTIVE: To test the effects of hyperoxia on bacterial translocation and mortality during gut-derived sepsis in a clinically relevant model of infection. METHODS: Balb/c mice were gavaged with 10(9) Escherichia coli and subjected to a 20% burn injury. Then, the animals were randomized to receive hyperoxia for different periods of time. Survival and the extent of translocation were determined, as well as intestinal histologic features. RESULTS:
Hyperoxia treatment preserved gut morphology and improved gut barrier function, decreasing the amount of bacterial translocation. Short-term (4- or 8-hour) hyperoxia (100% oxygen) treatment improved survival only on day 1 after injury but did not affect the final outcome. Short-term (8-hour) hyperoxia (100% oxygen) plus 5-day 40% oxygen environment significantly improved long-term survival. CONCLUSION: Tissue pO2 may be an important regulator of gut barrier function. Hyperoxia treatment appears to play a major role in preserving gut barrier function.
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Authors | R Gennari, J W Alexander |
Journal | Archives of surgery (Chicago, Ill. : 1960)
(Arch Surg)
Vol. 131
Issue 1
Pg. 57-62
(Jan 1996)
ISSN: 0004-0010 [Print] United States |
PMID | 8546578
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Animals
- Bacterial Translocation
(drug effects, physiology)
- Mice
- Mice, Inbred BALB C
- Oxygen
(therapeutic use)
- Sepsis
(microbiology, therapy)
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