Establishment and characterization of a novel human monocytic leukemia cell line (KP-1) expressing scavenger receptor.

Abstract

A new monocytic leukemia cell line (KP-1) was established from a 2-y-old Japanese girl with acute monocytic leukemia. The KP-1 cells were maintained in suspension culture with a doubling time of 96 h. The cells were positively stained with alpha-naphtyl butyrate esterase, but not with naphthol AS-D chloroacetate esterase, myeloperoxidase, and periodic acid-Schiff reagent. Cell surface marker analysis revealed that the cells were CD4, CD11a, CD11c, CD13, CD14, CD18, CD33, and HLA-DR positive. Karyotype analysis revealed near diploidy (47 XX) and a translocation t(11;19) was found. When treated with 12-o-tetradecanoylphorbol 13-acetate, KP-1 cells became tightly adherent, showed the enhanced reactivity for alpha-naphtyl butyrate esterase, and produced several monokines such as IL-1 beta, tumor necrosis factor-alpha, and macrophage colony-stimulating factor. Immunoelectron microscopy demonstrated that the human macrophage scavenger receptor was expressed after 12-o-tetradecanoylphorbol-13-acetate treatment, and the cells accumulated a large amount of cholesterol esters in the presence of acetylated LDL. Compared with another human monocytic leukemia cell line, THP-1, KP-1 expressed scavenger receptor and accumulated cholesterol ester more rapidly in the presence of 12-o-tetradecanoyl phorbol-13-acetate and acetylated LDL. Scatchard analysis using 125I-labeled acetylated LDL revealed a typical saturation curve with an apparent kd of 1.7 x 10(-7) M and 3400 binding sites per cell. KP-1 retained the characteristics of monocyte-macrophage lineage cells and will facilitate the in vitro studies of the pathologic and physiologic roles of scavenger receptors.

• Authors: N Adachi, K Takaki, R Nakamura, T Ohta, M Naito, S Horiuchi, I Matsuda
• Journal: Pediatric research (Pediatr Res) Vol. 34 Issue 3 Pg. 258-64 (Sep 1993) ISSN: 0031-3998 [Print] United States
• PMID: 8134164 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Neoplasm
• Biomarkers, Tumor
• Cholesterol Esters
• Lipoproteins, LDL
• Membrane Proteins
• Monokines
• Neoplasm Proteins
• Receptors, Immunologic
• Receptors, Lipoprotein
• Receptors, Scavenger
• Scarb1 protein, mouse
• Scavenger Receptors, Class B
• Tetradecanoylphorbol Acetate

Topics

• Antigens, Neoplasm (analysis)
• Biomarkers, Tumor (analysis)
• Cell Differentiation (drug effects)
• Child, Preschool
• Cholesterol Esters (metabolism)
• Chromosome Aberrations
• Female
• Gene Expression Regulation, Leukemic (drug effects)
• Humans
• Karyotyping
• Leukemia, Monocytic, Acute (genetics, metabolism, pathology)
• Lipoproteins, LDL (pharmacology)
• Macrophages (metabolism, pathology)
• Membrane Proteins
• Microscopy, Immunoelectron
• Monokines (biosynthesis)
• Neoplasm Proteins (biosynthesis)
• Neoplastic Stem Cells (drug effects, metabolism, ultrastructure)
• Phagocytosis
• Receptors, Immunologic (biosynthesis)
• Receptors, Lipoprotein
• Receptors, Scavenger
• Scavenger Receptors, Class B
• Tetradecanoylphorbol Acetate (pharmacology)
• Tumor Cells, Cultured (metabolism)