Abstract |
S100-positive, T cell chronic lymphoproliferative disease (S100-CLPD) is a rare and aggressive hematologic disorder in which the cytoplasmic protein S100 is expressed at high levels in abnormal lymphocytes. Using a DNA probe specific for human herpesvirus 6 (HHV-6), 2 cases of S100-CLPD were examined by DNA and RNA hybridization analysis. The results indicated that HHV-6 DNA was present in uncultured peripheral blood mononuclear cells (PBMC) and that a 1.3-kb viral transcript was expressed in both cases. Analysis of flow-sorted PBMC from 1 case demonstrated that HHV-6 DNA was present exclusively in the S100-positive fraction. Studies using other HHV-6 DNA probes suggested infection with HHV-6 variant B in both cases. Hybridization studies using DNA from PBMC of 27 cases of T cell chronic lymphoproliferative disease of other types showed no evidence of HHV-6. These studies suggest a possible specific association of HHV-6 with the unusual disorder S100-CLPD.
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Authors | D K Braun, P E Pellett, C A Hanson |
Journal | The Journal of infectious diseases
(J Infect Dis)
Vol. 171
Issue 5
Pg. 1351-5
(May 1995)
ISSN: 0022-1899 [Print] United States |
PMID | 7751716
(Publication Type: Case Reports, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- DNA, Viral
- RNA, Viral
- S100 Proteins
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Topics |
- Adult
- DNA, Viral
(blood)
- Female
- Herpesviridae Infections
(virology)
- Herpesvirus 6, Human
(isolation & purification)
- Humans
- Leukocytes, Mononuclear
(virology)
- Lymphoproliferative Disorders
(virology)
- Male
- Middle Aged
- Nucleic Acid Hybridization
- RNA, Viral
(blood)
- S100 Proteins
(analysis)
- T-Lymphocytes
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