Abstract | BACKGROUND: STUDY DESIGN AND METHODS: A reverse dot blot technique employing polymerase chain reaction-amplified genomic DNA was applied in genotyping the five major human platelet antigens in the following populations: 100 African American and 100 white women admitted to the obstetric unit at Johns Hopkins Hospital (Baltimore, MD) and 100 inpatients at Yonsei University (Seoul, Korea). RESULTS: The gene frequencies of HPA-2b (Koa) and HPA-5b (Bra) in African Americans were twice those in whites (African Americans: 0.18 and 0.21, respectively; whites: 0.09 and 0.11, respectively). There is a very low gene frequency of the HPA-1b (PIA2) allele in Koreans (0.005). No significant differences were found in the gene frequencies of the human platelet antigens in whites in this series and those in published European studies. CONCLUSION: These studies indicate a higher potential risk for alloimmunization to HPA-2 (Ko) and HPA-5 (Br) antigens in African Americans than in whites. In addition, the low gene frequency of HPA-1b (PIA2) in African Americans and Koreans suggests that alloimmunization to HPA-1a (PIA1) would be very unusual in these populations. These data may provide the basis for planning neonatal alloimmune thrombocytopenia screening programs in certain ethnic populations.
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Authors | H O Kim, Y Jin, T S Kickler, K Blakemore, O H Kwon, P F Bray |
Journal | Transfusion
(Transfusion)
Vol. 35
Issue 10
Pg. 863-7
(Oct 1995)
ISSN: 0041-1132 [Print] United States |
PMID | 7570918
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antigens, Human Platelet
- DNA
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Topics |
- Antigens, Human Platelet
(genetics)
- Asian People
(genetics)
- Black People
(genetics)
- DNA
(analysis)
- Female
- Gene Frequency
- Genotype
- Humans
- Korea
- Nucleic Acid Hybridization
- Polymerase Chain Reaction
- United States
- White People
(genetics)
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