Abstract |
An assay for the estimation of guanosine deaminase is described. The method employs guanosine as substrate and after incubation of serum and substrate at 22 degrees C for 18 h the ammonia liberated is estimated using the Berthelot reaction. Absorbance is measured as 625 nm and the catalytic activity read from a standard curve obtained using ammonia standards. The method provides reproducible measurements of serum guanosine deaminase. The results obtained using 'normal' sera have been used to calculate the 'normal range' for the enzyme in serum. Preliminary results suggest that guanosine deaminase is increased in hepatitis and in patients with liver metastases but normal in all other liver diseases including cirrhosis and obstructive jaundice.
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Authors | D D Jones, E L Roberts, A G Davies |
Journal | Journal of clinical chemistry and clinical biochemistry. Zeitschrift fur klinische Chemie und klinische Biochemie
(J Clin Chem Clin Biochem)
Vol. 21
Issue 12
Pg. 835-40
(Dec 1983)
ISSN: 0340-076X [Print] Germany |
PMID | 6663243
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Indicators and Reagents
- Aminohydrolases
- Nucleoside Deaminases
- guanosine deaminase
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Topics |
- Aminohydrolases
(blood)
- Clinical Enzyme Tests
- Humans
- Indicators and Reagents
- Kinetics
- Liver Diseases
(diagnosis, enzymology)
- Nucleoside Deaminases
- Reference Values
- Spectrophotometry
(methods)
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