Cytomegalovirus Glycoprotein B Genotype in Patients with Anterior Segment Infection.

Abstract

(1) The glycoprotein B (gB) on the viral envelope, encoded by the most widely characterised polymorphic gene, gpUL55, is responsible for cytomegalovirus (CMV) entry into the host and could serve as a potential marker of pathogenicity. The aim of the present study is to investigate the distribution of the CMV gB genotype in anterior segment infection in Taiwan and its correlation with clinical manifestations and outcomes. (2) Fifty-seven patients with CMV anterior segment infection were identified according to clinical features and positivity for CMV DNA in aqueous humour samples. CMV gB genotyping was performed through polymerase chain reaction assays. Patients' medical records were retrospectively reviewed. (3) Among the 57 aqueous humour samples tested for gB, 40 (70.28%) had multiple gB genotypes, and only 17 (29.82%) had a single gB genotype. Compared with single-genotype infection, multiple-genotype infection was correlated with higher CMV loads (p < 0.001) but not correlated with outcome. A higher proportion of patients with the gB3 genotype had received filtering surgery before antiviral treatment than those without the gB3 genotype (p = 0.046). (4) Multiple-genotype infection was highly prevalent in CMV anterior segment infection in Taiwan, and gB1 and gB3 were predominant. Multiple-genotype infection was correlated with higher CMV loads but not with specific clinical manifestations or prognostic outcomes. The gB3 genotype may be correlated with poor intraocular pressure control.

• Authors: Chu-Yen Huang, Yu-Chun Cheng, Yih-Shiou Hwang, Eugene Yu-Chuan Kang, Ching-Hsi Hsiao
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 24 Issue 7 (Mar 27 2023) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 37047276 (Publication Type: Journal Article)

Chemical References

• glycoprotein B, Simplexvirus
• Viral Envelope Proteins
• DNA, Viral

Topics

• Humans
• Cytomegalovirus (genetics)
• Retrospective Studies
• Viral Envelope Proteins (genetics)
• Cytomegalovirus Infections (genetics)
• Genotype
• DNA, Viral (genetics, analysis)