Abstract | BACKGROUND: METHODS: RESULTS: F2RL1 was substantially up-regulated in LUAD tissues and cells, and overexpression of F2RL1 could promote proliferation and stem cell sphere-forming of lung cancer cell lines, as well as formation of blood vessels and branch points of human umbilical vein endothelial cells (HUVECs). Meanwhile, overexpression of F2RL1 significantly upregulated VEGFA expression and promoted EGFR phosphorylation. EGFR inhibitor AG1478 treatment significantly down-regulated pEGFR, and AG1478 treatment reversed the promoting effect of cancer cell cultured medium (oe-F2RL1) on HUVEC angiogenesis. SIGNIFICANCE: In summary, this study revealed the molecular mechanism of PAR2 promoting LUAD angiogenesis by activating EGFR signaling pathway, which further improves our understanding of LUAD angiogenesis, and provides a potential therapeutic strategy for LUAD treatment.
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Authors | Yong Li, Huiqin Huang, Xiaoyun Chen, Nanding Yu, Xiangli Ye, Limin Chen, Zhenghui Huang |
Journal | Tissue & cell
(Tissue Cell)
Vol. 79
Pg. 101918
(Dec 2022)
ISSN: 1532-3072 [Electronic] Scotland |
PMID | 36148733
(Publication Type: Journal Article)
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Copyright | Copyright © 2022 Elsevier Ltd. All rights reserved. |
Chemical References |
- RTKI cpd
- Receptor, PAR-2
- Vascular Endothelial Growth Factor A
- ErbB Receptors
- EGFR protein, human
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Topics |
- Humans
- Receptor, PAR-2
(genetics, metabolism)
- Vascular Endothelial Growth Factor A
(genetics, metabolism)
- Cell Movement
(genetics)
- Gene Expression Regulation, Neoplastic
- Cell Line, Tumor
- Adenocarcinoma of Lung
(pathology)
- Neovascularization, Pathologic
(genetics, pathology)
- Lung Neoplasms
(pathology)
- Cell Proliferation
(genetics)
- ErbB Receptors
(genetics, metabolism)
- Adenocarcinoma
(genetics, pathology)
- Human Umbilical Vein Endothelial Cells
(metabolism)
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