Long non-coding RNA LINC01793 as a potential diagnostic biomarker of hepatitis B virus-related hepatocellular carcinoma.

Abstract

BACKGROUND:

There is growing evidence that long non-coding RNAs (lncRNAs) play important roles in the progression of hepatocellular carcinoma (HCC) and may serve as diagnostic markers. This study investigates the diagnostic efficiency of the long intergenic non-protein-coding RNA 1793 (LINC01793) in hepatitis B virus (HBV)-related HCC.

METHODS:

Bioinformatics methods were used to screen the aberrantly expressed lncRNAs in HCC tissues based on The Cancer Genome Atlas (TCGA). Quantitative reverse transcription polymerase chain reaction was performed to assess the expression of the candidate lncRNAs in tissues, cells and whole blood samples of patients with HBV-related HCC, liver cirrhosis (LC), chronic hepatitis (CHB), and healthy controls. Then, the correlations between LINC01793 and clinical characteristics were analyzed. Finally, the diagnostic value of LINC01793 was explored based on the receiver operating characteristic curve.

RESULTS:

LINC01793 was remarkably upregulated in the HCC tissues and cells. It was highly expressed in the whole blood of the HBV-related HCC patients, unlike in that of the healthy controls and of the CHB and LC patients. Subsequent analysis revealed that high LINC01793 was related to the Barcelona Clinic Liver Cancer (P = 0.007), tumor invasion (P = 0.042), the number of tumors (P = 0.031) and serum level of alanine aminotransferase(p = 0.022). The areas under the curve of LINC01793, for distinguishing HCC from healthy controls, CHB and LC patients, were 0.824, 0.767 and 0.756, respectively. In addition, the combination of LINC01793 with alpha fetoprotein (AFP) had a stronger diagnostic value than LINC01793 or AFP alone in AFP-negative HCC patients.

CONCLUSION:

High expression of LINC01793 is correlated with adverse clinical characteristics and can serve as a non-invasive biomarker of HCC.

• Authors: Cuiju Mo, Junrong Wu, Jingzhe Sui, Yan Deng, Meng Li, Zhao Cao, Zuojian Hu, Junhui Huang, Shan Li
• Journal: Clinical biochemistry (Clin Biochem) Vol. 108 Pg. 56-62 (Oct 2022) ISSN: 1873-2933 [Electronic] United States
• PMID: 35760369 (Publication Type: Journal Article)
• Copyright: Copyright © 2022 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.

Chemical References

• Biomarkers
• Biomarkers, Tumor
• RNA, Long Noncoding
• alpha-Fetoproteins
• Alanine Transaminase

Topics

• Alanine Transaminase
• Biomarkers
• Biomarkers, Tumor (genetics)
• Carcinoma, Hepatocellular (diagnosis, genetics, pathology)
• Hepatitis B virus (genetics)
• Hepatitis B, Chronic (complications, genetics)
• Humans
• Liver Cirrhosis (diagnosis, genetics, pathology)
• Liver Neoplasms (diagnosis, genetics, pathology)
• RNA, Long Noncoding (genetics)
• ROC Curve
• alpha-Fetoproteins (analysis)