Fibroblast Growth Factor 3 Is Associated with Tongue Squamous Cell Carcinoma: A Controlled Study.

Abstract

Objective:

To explore the effects of fibroblast growth factor 3 (FGF3) on the proliferation, cell cycle, and apoptosis of the tongue squamous cell carcinoma SCC-9 cell line (SCC-9).

Methods:

We measured the proliferation of SCC-9 cells in a control group, an FGF3 intervention group, and a fibroblast growth factor (FGFR) inhibitor intervention group in cholecystokinin octapeptide (CCK-8) experiments. We studied effects of FGF3 on the cell cycle and apoptosis of tongue cancer cells using flow cytometry. We further explored the IRS1/PI3K/AKT signaling pathway by measuring BCL-2 and Bcl-2 Associated X-protein (BAX) mRNA and protein levels with RT-PCR and western blot, respectively.

Results:

Results from the CCK-8 experiment showed that survival rates of cells in the control group, FGF3 intervention group, and FGFR inhibitor intervention group were 100.000% ± 4.026%, 136.330% ± 9.779%, and 83.199% ± 4.954%, respectively; survival rates of SCC-9 cells in all three groups were statistically significant (P < 0.05). Compared with that in the control group, the ratio of cells in G0/G1 phase in the FGFR inhibitor intervention group was higher (P < 0.05) and that in G2/M phase was lower, while the FGF3 intervention group showed opposite results (P < 0.05). The apoptosis rate of tongue cancer cells differed significantly between the FGFR inhibitor intervention and the control groups (P < 0.05). The mRNA and protein expression levels of IRS1, PI3K, and BCL-2 were all increased in the FGF3 intervention group (P < 0.05), while BAX mRNA and protein expression levels were decreased (P < 0.05). The mRNA expression levels of protein kinase B (AKT) showed no differences between groups. The p-AKT protein was overexpressed, while the total amount of AKT protein remained stable (P < 0.05).

Conclusion:

FGF3 contributes to the proliferation of SCC-9 cells by increasing the proportion of cells in G2/M phase. Therefore, appropriately timed inhibition of FGF3 can potentially promote tumor apoptosis through the IRS1/PI3K/AKT signaling pathway. Our results demonstrate the role of FGF3 in the tumor microenvironment in tongue squamous cell carcinoma SCC-9 cells and suggest new therapeutic targets.

• Authors: Yang Zhang, Pan Liu, Weipeng Su, Gaowa Aodeng, Huarong Zhao
• Journal: Computational and mathematical methods in medicine (Comput Math Methods Med) Vol. 2022 Pg. 3331119 ( 2022) ISSN: 1748-6718 [Electronic] United States
• PMID: 35720042 (Publication Type: Journal Article, Retracted Publication)
• Copyright: Copyright © 2022 Yang Zhang et al.

Chemical References

• FGF3 protein, human
• Fibroblast Growth Factor 3
• Proto-Oncogene Proteins c-bcl-2
• RNA, Messenger
• bcl-2-Associated X Protein
• Proto-Oncogene Proteins c-akt
• Sincalide

Topics

• Apoptosis (genetics)
• Carcinoma, Squamous Cell (metabolism)
• Cell Line, Tumor
• Cell Proliferation (genetics)
• Fibroblast Growth Factor 3 (pharmacology)
• Humans
• Phosphatidylinositol 3-Kinases (metabolism)
• Proto-Oncogene Proteins c-akt (metabolism)
• Proto-Oncogene Proteins c-bcl-2 (genetics, pharmacology, therapeutic use)
• RNA, Messenger (genetics)
• Sincalide (pharmacology, therapeutic use)
• Tongue
• Tongue Neoplasms (drug therapy, genetics, pathology)
• Tumor Microenvironment
• bcl-2-Associated X Protein (pharmacology)