Abstract | PURPOSE: METHODS: Subcutaneous and intraperitoneal tumors, and metastasis models were established in immune-deficient BALB/c nude and BALB/c Rag-2/Jak3 double-deficient (BRJ) mice using the human neuroblastoma cell lines IMR-32, LA-N-2, or NB-69. For evaluating polyphyllin D activity, we used a mouse model of liver metastasis with the IMR-32 cells line injected through the tail vein. We analyzed the livers number and area of liver tumors in of the phosphate buffer solution- and polyphyllin D-treated groups. RESULTS: Liver metastasis and intraperitoneal dissemination models were successfully established in immune-deficient BRJ mice using the three human neuroblastoma cell lines. In the liver metastasis, the model of IMR-32 cells, we found that polyphyllin D suppressed both the number and total area of metastatic foci the average number of metastatic foci, average focus areas, and number of cleaved caspase-3-positive cells were significantly lower in the polyphyllin D group (p = 0.016, 0.020, 0.043, respectively). CONCLUSIONS:
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Authors | Yasuhiro Kondo, Shunsuke Watanabe, Atsuki Naoe, Toshiyuki Takeuchi, Atsuko Niimi, Motoshi Suzuki, Naoya Asai, Seiji Okada, Tomonori Tsuchiya, Mika Murayama, Toshihiro Yasui, Mikihiro Inoue, Tatsuya Suzuki |
Journal | Pediatric surgery international
(Pediatr Surg Int)
Vol. 38
Issue 8
Pg. 1157-1163
(Aug 2022)
ISSN: 1437-9813 [Electronic] Germany |
PMID | 35699751
(Publication Type: Journal Article)
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Copyright | © 2022. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature. |
Chemical References |
- Saponins
- polyphyllin D
- Diosgenin
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Topics |
- Animals
- Apoptosis
- Cell Line, Tumor
- Diosgenin
(analogs & derivatives, pharmacology)
- Liver Neoplasms
(drug therapy)
- Mice
- Neuroblastoma
(drug therapy, pathology)
- Saponins
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