Capsaicin Alleviates Vascular Endothelial Dysfunction and Cardiomyopathy via TRPV1/eNOS Pathway in Diabetic Rats.

Abstract

Background:

Endothelial dysfunction and cardiomyopathy are considered to be important vascular complications associated with diabetes. This study was designed to investigate whether capsaicin (CAP), a selective TRPV1 agonist, could prevent diabetes-induced endothelial dysfunction and cardiomyopathy.

Methods:

Male Sprague Dawley rats aged 8 weeks were injected intraperitoneally with streptozotocin (STZ, 50 mg/kg) to establish the diabetes model. The diabetic rats were randomly divided into the untreated diabetes group (DM, 10/group) and diabetes plus CAP treatment group (DM+CAP, 10/group); meanwhile, the nondiabetic healthy rats were used as normal controls (10/group). DM+CAP group were treated with CAP by gavage for 8 weeks. The cultured mouse vascular endothelial cells were exposed to different concentrations of glucose in the presence or absence of CAP treatment. The TRPV1 inhibitor capsazepine (CPZ) and eNOS inhibitor L-NAME were used in vivo and in vitro experiment.

Results:

CAP treatment significantly decreased the serum total cholesterol (TC) and total triglyceride (TG) and ameliorated the pathogenesis and fibrosis in the heart, while did not significantly improve plasma glucose level and the body weights of diabetic rats. In addition, CAP enhanced the expression of TRPV1 and eNOS in the heart and normalized the vascular permeability under diabetic state. Similarly, CAP treatment also increased nitric oxide and reduced reactive oxygen species. The same results were observed in cultured mouse vascular endothelial cells by CAP treatment. These beneficial effects of CAP were abolished by either CPZ or L-NAME.

Conclusions:

CAP might protect against hyperglycemia-induced endothelial dysfunction and diabetic cardiomyopathy through TRPV1/eNOS pathway.

• Authors: Qiuyue Wang, Caihui Zhang, Chen Yang, Yue Sun, Keyang Chen, Yao Lu
• Journal: Oxidative medicine and cellular longevity (Oxid Med Cell Longev) Vol. 2022 Pg. 6482363 ( 2022) ISSN: 1942-0994 [Electronic] United States
• PMID: 35602097 (Publication Type: Journal Article, Retracted Publication)
• Copyright: Copyright © 2022 Qiuyue Wang et al.

Chemical References

• TRPV Cation Channels
• TRPV1 protein, mouse
• Trpv1 protein, rat
• Streptozocin
• Nitric Oxide Synthase Type III
• Capsaicin
• NG-Nitroarginine Methyl Ester

Topics

• Animals
• Capsaicin (pharmacology, therapeutic use)
• Diabetes Mellitus, Experimental (metabolism)
• Diabetic Cardiomyopathies (metabolism)
• Endothelial Cells (metabolism)
• Endothelium, Vascular (metabolism)
• Male
• Mice
• NG-Nitroarginine Methyl Ester (pharmacology)
• Nitric Oxide Synthase Type III (metabolism)
• Rats
• Rats, Sprague-Dawley
• Streptozocin (pharmacology)
• TRPV Cation Channels (metabolism)
• Vascular Diseases (pathology)