Tanshinone IIA Alleviates the Biological Characteristics of Colorectal Cancer via Activating the ROS/JNK Signaling Pathway.

Abstract

BACKGROUND:

Tanshinone IIA (Tan IIA) exerts a significant inhibitory effect on various tumor cells since it induces cell apoptosis and affects the proliferation, differentiation, metastasis, and invasion of tumor cells. However, the mechanism underlying the antitumor activity of Tan IIA has not been totally elucidated.

OBJECTIVE:

This study aimed to uncover the role of Tan IIA in colorectal cancer (CRC) and its potential mechanism of action.

METHODS:

Cell proliferation was assessed using CCK-8 and colony formation assays. Western blot analysis was carried out to detect the expression of related proteins. Cell apoptosis was assessed using flow cytometry. Furthermore, tumor size and tumor weight of CRC xenograft mice were recorded before and after Tan IIA treatment. The production of reactive oxygen species (ROS) was measured by a ROS kit.

RESULTS:

The results revealed that Tan IIA induced autophagy and apoptosis via activating the ROS/JNK signaling pathway in CRC cells, thus inhibiting the progression of CRC in vivo.

CONCLUSION:

The aforementioned findings indicated that Tan IIA exerted an antiproliferative effect on CRC by inducing cell autophagy and apoptosis via activating the ROS/JNK signaling pathway. Therefore, Tan IIA may be considered a potential therapeutic agent for treating CRC.

• Authors: Jun Qian, Yi Cao, Junfeng Zhang, Lingchang Li, Juan Wu, Jialin Yu, Jiege Huo
• Journal: Anti-cancer agents in medicinal chemistry (Anticancer Agents Med Chem) Vol. 23 Issue 2 Pg. 227-236 ( 2023) ISSN: 1875-5992 [Electronic] Netherlands
• PMID: 35593352 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright© Bentham Science Publishers; For any queries, please email at [email protected].

Chemical References

• tanshinone
• Reactive Oxygen Species

Topics

• Humans
• Animals
• Mice
• MAP Kinase Signaling System
• Reactive Oxygen Species (metabolism)
• Cell Line, Tumor
• Colorectal Neoplasms (pathology)
• Apoptosis