Ratiometric imaging of butyrylcholinesterase activity in mice with nonalcoholic fatty liver using an AIE-based fluorescent probe.

Abstract

Butyrylcholinesterase (BChE) is an essential human biomarker which is related to liver and neurodegenerative diseases. It is of great significance to develop a fluorescent probe that can image BChE in vitro and in vivo. Unfortunately, most fluorescent probes that are based on a single change in fluorescence intensity are susceptible to environmental interference. Therefore, we reported an easily available ratiometric fluorescent probe, TB-BChE, with aggregation-induced emission (AIE) characteristics for ratiometric imaging of BChE. TB-BChE demonstrated excellent sensitivity (LOD = 39.24 ng mL-1) and specificity for BChE. Moreover, we have successfully studied the ratiometric imaging of TB-BChE to BChE in a nonalcoholic fatty liver disease model. These results indicated that TB-BChE is expected to become a powerful analysis tool for butyrylcholinesterase research in basic medicine and clinical applications.

• Authors: Chunbai Xiang, Jingjing Xiang, Xing Yang, Chunbin Li, Lihua Zhou, Daoyong Jiang, Yonglin Peng, Zhen Xu, Guanjun Deng, Baode Zhu, Pengfei Zhang, Lintao Cai, Ping Gong
• Journal: Journal of materials chemistry. B (J Mater Chem B) Vol. 10 Issue 22 Pg. 4254-4260 (06 08 2022) ISSN: 2050-7518 [Electronic] England
• PMID: 35583194 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fluorescent Dyes
• Butyrylcholinesterase

Topics

• Animals
• Butyrylcholinesterase
• Diagnostic Imaging
• Fluorescent Dyes
• Mice
• Non-alcoholic Fatty Liver Disease (diagnostic imaging)