Modern dairy cattle suffer from increased incidence and severity of
mastitis during major physiological transitions of the lactation cycle. Oxidative stress, a condition resulting from inadequate
antioxidant defense against reactive
oxygen and
nitrogen species, is a major underlying component of
mastitis pathophysiology.
Isoprostanes (IsoP) are molecules derived from cellular
lipid membranes upon non-enzymatic interaction with reactive species during
inflammation, and are regarded as highly sensitive and specific
biomarkers of oxidative stress. Changes in IsoP concentrations have been noted during major physiological transitions and diseases such as coliform
mastitis in dairy cattle. However, the
biological role of IsoP during oxidative stress in dairy cows has not been well-elucidated. Therefore, this study aimed to characterize the impacts of IsoP on oxidative stress outcomes in a bovine model of acute endothelial
inflammation. Bovine aortic endothelial cells (BAEC; n = 4) were stimulated with 2,2'-azobis (2-amidinopropane) dihydrochloride (
AAPH) or
lipopolysaccharide (LPS) with or without
15-F2t-IsoP to determine how IsoP influence oxidative stress outcomes. Our endothelial
inflammation model showed relatively decreased reactive metabolites and increased barrier integrity in cells treated with both the agonist and IsoP compared to agonist treatment alone. However, IsoP do not appear to affect oxidative stress outcomes during acute
inflammation. Understanding the effect of IsoP on BAEC is an early step in elucidating how IsoP impact dairy cows during times of oxidative stress in the context of acute clinical
mastitis. Future studies should define the optimal dosing and treatment timing of IsoP to maximize their cytoprotective potential during acute
inflammation.