Abstract |
The aim of this study was to investigate the efficacy of a skin substitute composed of mPEG-PCL-grafted- gelatin (Bio-Syn)/ hyaluronan/ chondroitin sulfate/ sericin and to study its in vitro biocompatibility with human fibroblasts, human keratinocytes and hMSCs in terms of cellular adhesion and proliferation (∼5-6 fold). mPEG-PCL was grafted into a gelatin backbone via a Michael addition reaction to prepare Bio-Syn and it was characterized using ATR-FTIR, 1H NMR and TNBS assay. Additionally, keratinocyte-hMSC contact co-culture studies showed that Bio-Syn composite scaffolds loaded with sericin promote hMSCs' epithelial differentiation with regard to qRT-PCR gene expression (ΔNp63α and keratin 14) and expression of various epithelial markers (Pan- cytokeratin, ΔNp63α and keratin 14). In vivo efficacy studies on a 2nd degree burn wound model in Wistar rats showed an improved rate of wound contraction, histology (H&E and Van Gieson's staining) and pro-healing marker ( hexosamine, hydroxyproline, etc.) expression in granular tissue compared to using the commercial dressing Neuskin™ and a cotton gauze control.
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Authors | Sirsendu Bhowmick, A V Thanusha, Arun Kumar, Dieter Scharnweber, Sandra Rother, Veena Koul |
Journal | RSC advances
(RSC Adv)
Vol. 8
Issue 30
Pg. 16420-16432
(May 03 2018)
ISSN: 2046-2069 [Electronic] England |
PMID | 35540513
(Publication Type: Journal Article)
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Copyright | This journal is © The Royal Society of Chemistry. |