Abstract | BACKGROUND: PURPOSE: METHODS: Methyl thiazolyl tetrazolium (MTT), colony formation and 5-ethynyl-2'-deoxyuridine (EDU) assays were performed to examine the effects of acetylshikonin in inhibiting the proliferation of NSCLC cells (PC-9, H1975 and A549). Scratch wound and transwell assays were used to evaluate the migration and invasion of NSCLC cells. Flow cytometry was employed to determine whether acetylshikonin could induce apoptosis. Proteome sequencing was used to identify the targets of acetylshikonin. Immunofluorescence staining and western blotting were utilized to verify the inhibition of STAT3 and EGFR phosphorylation. A xenotransplantation model was established to evaluate the efficacy of acetylshikonin in nude mice. RESULTS: Our data demonstrated that acetylshikonin significantly decreased the survival rate of human NSCLC cells, increased the apoptotic rate and inhibited cell migration dose-dependently. Immunofluorescence staining and western blotting analyses revealed that acetylshikonin inhibited EGFR and STAT3 pathways. Acetylshikonin also inhibited tumor growth in a xenograft model better than inhibitors of EGFR and STAT3. CONCLUSION:
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Authors | Yemeng Tang, Yanmao Wang, Xian Wang, Zhucheng Zhao, Haijian Cai, Mengyao Xie, Xintong Jiang, Luyao Zhang, Jiayun Cheng, Lehe Yang, Liangxing Wang, Chengguang Zhao, Xiaoying Huang |
Journal | Phytomedicine : international journal of phytotherapy and phytopharmacology
(Phytomedicine)
Vol. 101
Pg. 154109
(Jul 2022)
ISSN: 1618-095X [Electronic] Germany |
PMID | 35526322
(Publication Type: Journal Article)
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Copyright | Copyright © 2022. Published by Elsevier GmbH. |
Chemical References |
- Anthraquinones
- STAT3 Transcription Factor
- STAT3 protein, human
- EGFR protein, human
- ErbB Receptors
- acetylshikonin
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Topics |
- Animals
- Anthraquinones
- Apoptosis
- Carcinoma, Non-Small-Cell Lung
(pathology)
- Cell Line, Tumor
- Cell Proliferation
- ErbB Receptors
(metabolism)
- Humans
- Lung Neoplasms
(pathology)
- Mice
- Mice, Nude
- STAT3 Transcription Factor
(metabolism)
- Xenograft Model Antitumor Assays
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