Abstract | AIMS: This study aimed to evaluate the impact of a 12-week calorie-restricted diet and recreational sports training on gene expressions IL-15, ATROGIN-1 and MURF-1 in skeletal muscle of T2D patients. METHODS: Older adults with T2D (n = 39, 60 ± 6.0 years, BMI 33.5 ± 0.6 kg/m2) were randomly allocated to Diet+Soccer (DS), Diet+Running (DR) or Diet (D). The training sessions were moderate-to-high-intensity and performed 3 × 40 min/week for 12-weeks. Gene expression from vastus lateralis muscle obtained by qRT-PCR, dual-energy X-ray and fasting blood testing measurements were performed before and after 12-weeks. Statistical analysis adopted were two-way ANOVA and Paired t-test for gene expression, and RM-ANOVA test for the remainder variables. RESULTS: Total body weight was reduced in ~4 kg representing body fat mass in all groups after 12-weeks (P < 0.05). HbA1c values decreased in all groups post-intervention. Lipids profile improved in the training groups (P < 0.05) after 12-weeks. ATROGIN-1 and MURF-1 mRNA reduced in the DS (1.084 ± 0.14 vs. 0.754 ± 1.14 and 1.175 ± 0.34 vs. 0.693 ± 0.12, respectively; P < 0.05), while IL-15 mRNA increased in the DR (1.056 ± 0.12 vs. 1.308 ± 0.13; P < 0.05) after 12-weeks intervention. CONCLUSION:
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Authors | Diana Bento da Silva Soares, Samuel Katsuyuki Shinjo, Aritânia Sousa Santos, Joyce de Cassia Rosa de Jesus, Simon Schenk, Gabriela Salim de Castro, Edmar Zanoteli, Peter Krustrup, Maria Elizabeth Rossi da Silva, Maysa Vieira de Sousa |
Journal | Experimental gerontology
(Exp Gerontol)
Vol. 164
Pg. 111831
(07 2022)
ISSN: 1873-6815 [Electronic] England |
PMID | 35525396
(Publication Type: Journal Article, Randomized Controlled Trial, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2022 Elsevier Inc. All rights reserved. |
Chemical References |
- IL15 protein, human
- Interleukin-15
- Muscle Proteins
- RNA, Messenger
- Tripartite Motif Proteins
- FBXO32 protein, human
- SKP Cullin F-Box Protein Ligases
- TRIM63 protein, human
- Ubiquitin-Protein Ligases
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Topics |
- Aged
- Caloric Restriction
- Diabetes Mellitus, Type 2
(genetics, metabolism, therapy)
- Exercise
(physiology)
- Gene Expression
- Humans
- Interleukin-15
(biosynthesis, genetics)
- Muscle Proteins
(biosynthesis, genetics)
- Muscle, Skeletal
(metabolism, physiology)
- RNA, Messenger
(genetics, metabolism)
- SKP Cullin F-Box Protein Ligases
(biosynthesis, genetics)
- Tripartite Motif Proteins
(biosynthesis, genetics)
- Ubiquitin-Protein Ligases
(biosynthesis, genetics)
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