Overexpression of miRNA-93-5p Promotes Proliferation and Migration of Bladder Urothelial Carcinoma via Inhibition of KLF9.

Abstract

We focused on studying the effects of a key miRNA-mRNA axis in bladder urothelial carcinoma (BUC). Firstly, miRNAs and mRNAs differentially expressed in BUC were analyzed. Clinical information in the TCGA database was used for survival analysis, and the regulator of miRNA-93-5p was predicted. miRNA-93-5p and KLF9 mRNA expression were detected by qRT-PCR. Protein level detection and targeting measurement were, respectively, achieved by western blot and dual-luciferase approaches. The proliferative, invasive, and migratory abilities were tested through CCK-8, Transwell, and wound healing methods. Cell apoptosis in each group was detected through flow cytometry. As discovered, miRNA-93-5p level was markedly high in BUC cells while KLF9 expression was remarkably low. miRNA-93-5p overexpression promoted BUC cell abilities. Besides, miRNA-93-5p inhibited KLF9 expression. Furthermore, KLF9 overexpression dramatically attenuated such promotion on cancer cell abilities. On the whole, miRNA-93-5p/KLF9 axis facilitated BUC progression, offering a new potential target for BUC patients.

• Authors: Tao Li, Qingjiang Xu, Yongbao Wei, Rongcheng Lin, Zhiwei Hong, Rong Zeng, Weilie Hu, Xiang Wu
• Journal: Computational and mathematical methods in medicine (Comput Math Methods Med) Vol. 2022 Pg. 8911343 ( 2022) ISSN: 1748-6718 [Electronic] United States
• PMID: 35495878 (Publication Type: Journal Article, Retracted Publication)
• Copyright: Copyright © 2022 Tao Li et al.

Chemical References

• KLF9 protein, human
• Kruppel-Like Transcription Factors
• MIRN93 microRNA, human
• MicroRNAs
• RNA, Messenger

Topics

• Carcinoma, Transitional Cell
• Cell Proliferation (genetics)
• Female
• Humans
• Kruppel-Like Transcription Factors (genetics)
• Male
• MicroRNAs (genetics, metabolism)
• RNA, Messenger (genetics)
• Urinary Bladder
• Urinary Bladder Neoplasms (genetics)