Fungal
keratitis is a corneal
infection, which severely impairs vision. The fungal pathogen provokes host immune response, but the excessive inflammatory response causes significant collateral damage to the cornea.
Eugenol, the main component of
clove oil, has been found to have a broad range of pharmacological activities including anti-microbial, antioxidation and anti-
inflammation. However, the role of
eugenol in Aspergillus fumigatus (A. fumigatus)
keratitis is unknown. In this study, we demonstrated that
eugenol reduced mice
keratitis severity, inflammatory cells infiltration, pro-inflammatory
cytokine expression, and the fungal load.
Eugenol also decreased the expressions of pro-inflammatory
cytokines in human corneal epithelial cells (HCECs). We confirmed that the anti-inflammatory effects of
eugenol were related to activating nuclear factor erythroid 2-related factor 2/
Heme Oxygenase-1 (Nrf2/HO-1) signaling pathway. Moreover, we demonstrated that
eugenol could inhibit the A. fumigatus growth and adhesion to host cells, as well as damage the fungal biofilm. The antifungal mechanism seemed to be disrupting the integrity of the fungal membrane and reducing the biosynthesis of
ergosterol. Taken together, our research suggested that
eugenol exerted protective effects on mouse A. fumigatus
keratitis, due to its anti-inflammatory and antifungal activity.