Abstract |
Tight junctions (TJs) of brain microvascular endothelial cells (BMECs) play a pivotal role in maintaining the blood-brain barrier (BBB) integrity; however, precise regulation of TJs stability in response to physiological and pathological stimuli remains elusive. Here, using RNA immunoprecipitation with next-generation sequencing (RIP-seq) and functional characterization, we identify SNHG12, a long non-coding RNA ( lncRNA), as being critical for maintaining the BBB integrity by directly interacting with TJ protein occludin. The interaction between SNHG12 and occludin is oxygen adaptive and could block Itch (an E3 ubiquitin ligase)-mediated ubiquitination and degradation of occludin in human BMECs. Genetic ablation of endothelial Snhg12 in mice results in occludin reduction and BBB leakage and significantly aggravates hypoxia-induced BBB disruption. The detrimental effects of hypoxia on BBB could be alleviated by exogenous SNHG12 overexpression in brain endothelium. Together, we identify a direct TJ modulator lncRNA SNHG12 that is critical for the BBB integrity maintenance and oxygen adaption.
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Authors | Yuan Li, Jia-Yi Wei, Hui Liu, Kang-Ji Wang, Sheng-Nan Jin, Zheng-Kang Su, Hui-Jie Wang, Jun-Xiu Shi, Bo Li, De-Shu Shang, Wen-Gang Fang, Xiao-Xue Qin, Wei-Dong Zhao, Yu-Hua Chen |
Journal | Cell reports
(Cell Rep)
Vol. 39
Issue 2
Pg. 110656
(04 12 2022)
ISSN: 2211-1247 [Electronic] United States |
PMID | 35417709
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved. |
Chemical References |
- Occludin
- RNA, Long Noncoding
- Ubiquitin-Protein Ligases
- Oxygen
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Topics |
- Animals
- Blood-Brain Barrier
(metabolism)
- Endothelial Cells
(metabolism)
- Hypoxia
(metabolism)
- Mice
- Occludin
(metabolism, pharmacology)
- Oxygen
(metabolism)
- RNA, Long Noncoding
(genetics, metabolism)
- Ubiquitin-Protein Ligases
(metabolism)
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