Diabetes results from insufficient production of the
hormone insulin from beta cells in pancreatic islets.
Islet transplantation can replace the lost beta cells in patients but is limited by the scarcity of available donor organs. Our aim is to differentiate pluripotent stem cells into functional islets that can serve as an unlimited source for
transplantation to treat diabetes. We have investigated the therapeutic potential of pancreatic endoderm cells derived from human embryonic stem cells. Several weeks following transplant into diabetic rodents, these cells mature and secrete sufficient human
insulin, in a regulated manner, to reverse diabetes. In rats, we observed inconsistent survival of pancreatic endoderm cells implanted subcutaneously in macroencapsulation devices designed to be immunoprotective via use of a cell impermeable layer, but this was rectified by the addition of portals designed to enable direct capillary vascular permeation into the device interior. In contrast both device types supported cell survival, differentiation and function in mice, with more rapid
C-peptide release and better
glucose tolerance observed using the devices containing portals. Kidney
capsule grafts often contained ductal cells and
cysts, whereas cells implanted subcutaneously within macroencapsulation devices differentiated predominantly to endocrine cells. In collaboration with Aspect Biosystems, we are also exploring the feasibility of using 3D bioprinting as an approach to both contain implanted islet cells and protect them from immune attack. As part of a ViaCyte clinical trial (clinicaltrial.gov identifier: NCT03163511), we investigated the safety and efficacy of pancreatic endoderm cells implanted in nonimmunoprotective macroencapsulation devices for the treatment of patients with
type 1 diabetes and
hypoglycemic unawareness. Patients underwent subcutaneous implant of cell products combined with an immunosuppressive regimen. After implant, patients had increased fasting Cpeptide levels, increased
glucose-responsive
C-peptide levels, and developed mixed meal-stimulated
C-peptide secretion. Patients had reduced
insulin requirements, increased time in target
blood glucose range, and improved
hypoglycemic awareness. Explanted grafts contained cells with a mature beta cell phenotype that were immunoreactive for
insulin,
islet amyloid polypeptide, and MAFA. Collectively, these findings support future investigation into optimizing cell
therapies for diabetes.