Abstract | BACKGROUND: METHODS: We measured the methylation levels of PRKCDBP in the three groups of NSCLC tissues. Promoter activity was measured by the dual luciferase assay, with 5'-aza-deoxycytidine to examine the effect of demethylation on the expression level of PRKCDBP. RESULTS: The methylation levels of PRKCDBP in tumor tissues and 3 cm para- tumor were higher than those of distant (>10 cm) non- tumor tissues. Receiver operating characteristic (ROC) curve analysis between tumor tissues and distant non- tumor tissues showed that the area under the line (AUC) was 0.717. Dual luciferase experiment confirmed that the promoter region was able to promote gene expression. Meanwhile, in vitro methylation of the fragment (PRKCDBP_Me) could significantly reduce the promoter activity of the fragment. Demethylation of 5'-aza-deoxycytidine in lung cancer cell lines A549 and H1299 showed a significant up-regulation of PRKCDBP mRNA levels. CONCLUSIONS:
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Authors | Jing Li, Lin Qi, Mingfang Zhang, Caiyun Yao, Jinan Feng, Zhonghua Zheng, Chujia Chen, Shiwei Duan, Yuanlin Qi |
Journal | Zhongguo fei ai za zhi = Chinese journal of lung cancer
(Zhongguo Fei Ai Za Zhi)
Vol. 25
Issue 2
Pg. 78-85
(Feb 20 2022)
ISSN: 1999-6187 [Electronic] China |
PMID | 35224960
(Publication Type: Journal Article)
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Chemical References |
- Biomarkers
- CAVIN3 protein, human
- Intracellular Signaling Peptides and Proteins
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Topics |
- Biomarkers
(metabolism)
- Carcinoma, Non-Small-Cell Lung
(pathology)
- Cell Line, Tumor
- DNA Methylation
- Gene Expression Regulation, Neoplastic
- Humans
- Intracellular Signaling Peptides and Proteins
(genetics)
- Lung Neoplasms
(pathology)
- Promoter Regions, Genetic
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