T Lymphocyte Infiltration in Association with IDO1 Expression in Resected Lung Adenocarcinoma and Normal Adjacent Lung Tissues.
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| Abstract | BACKGROUND:
Indoleamine 2,3-dioxygenase 1 (IDO1) catalyzes the first step of tryptophan catabolism in the kynurenine (Kyn) pathway. IDO1 downregulates natural killer cell receptors, and by mechanism, tumor cells escape immune surveillance. METHODS: IDO1 protein and mRNA were assessed by immunohistochemistry, immunoblotting, and PCR in the 68 resected lung adenocarcinomas at stages I-III as well as adjacent normal lung tissues. Infiltration of CD3, CD8, and CD4 lymphocytes in the tumor and adjacent normal lung tissues was assessed by immunohistochemical staining. RESULTS: IDO1 protein and mRNA were detected in various stages of lung adenocarcinoma with highest expression at stage III. In contrast, biomarkers of T cell subset, CD3, CD4, and CD8, were highly expressed in the normal lung tissues and stage I adenocarcinoma tissues but significantly reduced in the stage II and III tumor tissues. CONCLUSIONS: The current study demonstrated that the higher level of IDO1 expression in the lung adenocarcinoma was, the less infiltration of T lymphocytes was found in the tumors. Findings of this study indicated that IDO1 may contribute to the reduction of T lymphocyte infiltration into the lung adenocarcinoma.
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| Authors | Xiaoling Zhao, Yaran Li, Xu Yang, Xiaochong Zhang, Jing Xie, Shaoteng Li, Hongzhen Liu, Jun Guo, Lili He, Wei Chen, Dengxiang Liu |
| Journal | BioMed research international (Biomed Res Int) Vol. 2022 Pg. 2381018 ( 2022) ISSN: 2314-6141 [Electronic] United States |
| PMID | 35187162 (Publication Type: Journal Article) |
| Copyright | Copyright © 2022 Xiaoling Zhao et al. |
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