Lactobacillus paracasei KW3110 Prevents Inflammatory-Stress-Induced Mitochondrial Dysfunction in Mouse Macrophages.

Abstract

Lactobacillus paracasei KW3110 (KW3110) has anti-inflammatory effects, including the prevention of blue light exposure induced retinal inflammation and ageing-related chronic inflammation in mice. The mechanism involves the promotion of anti-inflammatory cytokine interleukin (IL)-10 production by KW3110, leading to reduced pro-inflammatory cytokine IL-1β production. Although various stress-induced mitochondrial damages are associated with excessive inflammatory responses, the effect of KW3110 on inflammatory-stress-induced mitochondrial damage remains unknown. In this study, we investigated the effect of KW3110 on inflammatory stress-induced mitochondrial damage using the murine macrophage-like cell line J774A.1. KW3110 treatment suppressed lipopolysaccharide (LPS)-induced mitochondrial dysfunction, including downregulation of membrane potential, induction of reactive oxygen species, and respiratory dysfunction. In addition, KW3110 prevented LPS-induced disruption of mitochondrial morphology including cristae structures. IL-10 treatment also ameliorated LPS-induced mitochondrial dysfunction and morphology disruption. These results suggest that KW3110 prevents LPS-induced mitochondrial dysfunction, potentially via promoting IL-10 production in mouse macrophages. We are the first to reveal a suppressive effect of lactic acid bacteria on mitochondrial morphology disruption in inflammatory-stressed macrophages. Our findings contribute to understanding inflammatory-stress-induced mitochondrial damage and developing food ingredients with preventive effects on mitochondrial-damage-derived inflammatory conditions.

• Authors: Takahiro Yamazaki, Sayuri Yamada, Konomi Ohshio, Miho Sugamata, Yuji Morita
• Journal: International journal of molecular sciences (Int J Mol Sci) Vol. 23 Issue 3 (Jan 27 2022) ISSN: 1422-0067 [Electronic] Switzerland
• PMID: 35163375 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• IL10 protein, mouse
• Lipopolysaccharides
• Interleukin-10

Topics

• Animals
• Anti-Inflammatory Agents (metabolism)
• Cell Line
• Gene Expression Regulation (drug effects)
• Interleukin-10 (metabolism)
• Lacticaseibacillus paracasei (physiology)
• Lipopolysaccharides (adverse effects)
• Macrophages (cytology, drug effects, metabolism)
• Mice
• Mitochondria (drug effects, metabolism)
• Oxidative Stress (drug effects)
• Probiotics
• Pyroptosis (drug effects)