Objectives:
DNA methyltransferases (DNMTs) and the
histone modification H3K9ac are epigenetic markers. This study aimed to describe the immunohistochemical expression of DNMT1, DNMT3A, DNMT3B, and H3K9ac in the dental follicle (DF),
ameloblastoma (
AME), and ameloblastic
carcinoma (AC), correlating these expressions with the recurrence and aggressive behavior in
ameloblastoma. Study Design: Immunohistochemical reactions were performed in 10 human DFs, 38
ameloblastomas, and 6 AC samples. Another 59
ameloblastomas assembled in a tissue microarray were used to compare the immunoexpression with the clinical, radiographic, and histopathological characteristics and the presence of BRAFv600e mutation. Each slide was digitized as a high-resolution image and quantified by Aperio ScanScope Nuclear V9 software. All statistical analyzes were performed using GraphPad Prism statistical software. Results: DNMT3B expression was higher in
ameloblastomas than in the DFs, while the AC overexpressed all
proteins. The
ameloblastomas with BRAFv600e mutation, vestibular/lingual, or vestibular/palatine bone cortical disruption and maxilla involvement showed DNMT1 overexpression, while recurrent cases had high DNMT3B levels. Conclusions: DNA methylation and
histone modification might play a role in the development, clinical aggressiveness, and recurrence rates of
ameloblastoma, such as the progression to AC. Further investigation about gene methylations in
ameloblastomas is needed to better understand its relationship with aggressiveness and recurrence.