Effects of delta-opioid receptor agonist pretreatment on the cardiotoxicity of bupivacaine in rats.

Abstract

BACKGROUND:

Delta-opioid receptor is widely expressed in human and rodent hearts, and has been proved to protect cardiomyocytes against ischemia/reperfusion and heart failure. The antagonist of delta-opioid receptor could block the rescue effect of lipid emulsion against local anesthetic cardiotoxicity. However, no evidence is available for the direct effect of delta-opioid-receptor agonists on the cardiotoxicity of local anesthetics.

METHODS:

Anesthetized Sprague Dawley rats were divided into five groups. Group NS received 2 ml·kg-1·min-1 normal saline, group LE received 2 ml·kg-1·min-1 30% lipid emulsion and group BW received 0.1, 1.0, or 5.0 mg/kg BW373U86, a delta-opioid-receptor agonist, for 5 min. Then 0.5% bupivacaine was infused intravenously at a rate of 3.0 mg·kg-1·min-1 until asystole. The time of arrhythmia, 50% mean arterial pressure-, 50% heart rate-reduction and asystole were recorded, and the dose of bupivacaine at each time point was calculated.

RESULTS:

All three different doses of BW373U86 did not affect the arrhythmia, 50% mean arterial pressure-reduction, 50% heart rate-reduction and asystole dose of bupivacaine compared with group NS. 30% LE significantly increased the bupivacaine threshold of 50% mean arterial pressure-reduction (17.9 [15.4-20.7] versus 7.2 [5.9-8.7], p = 0.018), 50% heart rate-reduction (18.7 ± 4.2 versus 8.8 ± 1.7, p < 0.001) and asystole (26.5 [21.0-29.1] versus 11.3 [10.7-13.4], p = 0.008) compared with group NS. There was no difference between group LE and group NS in the arrhythmia dose of bupivacaine (9.9 [8.9-11.7] versus 5.6 [4.5-7.0], p = 0.060).

CONCLUSIONS:

Our data show that BW373U86 does not affect the cardiotoxicity of bupivacaine compared with NS control in rats. 30% LE pretreatment protects the myocardium against bupivacaine-induced cardiotoxicity.

• Authors: Chenran Wang, Shen Sun, Jing Jiao, Xinhua Yu, Shaoqiang Huang
• Journal: BMC anesthesiology (BMC Anesthesiol) Vol. 22 Issue 1 Pg. 19 (01 12 2022) ISSN: 1471-2253 [Electronic] England
• PMID: 35021986 (Publication Type: Journal Article)
• Copyright: © 2022. The Author(s).

Chemical References

• Anesthetics, Local
• Benzamides
• Piperazines
• Receptors, Opioid
• BW 373U86
• Bupivacaine

Topics

• Anesthetics, Local (adverse effects)
• Animals
• Benzamides (pharmacology)
• Bupivacaine (adverse effects)
• Cardiotoxicity (prevention & control)
• Disease Models, Animal
• Dose-Response Relationship, Drug
• Piperazines (pharmacology)
• Rats
• Rats, Sprague-Dawley
• Receptors, Opioid (agonists)