Abstract | BACKGROUND AND OBJECTIVE: METHODS: Mice were randomly divided into sham, MI + normal saline (NS) and MI + STS (20.8 mg/kg/day intraperitoneally) groups. MI was established following left anterior descending artery ligation. Cardiac function was evaluated using echocardiography. Scar size and myocardial fibrosis-associated markers were detected using Masson's trichrome staining and western blot analysis (WB). Necrosis and inflammation were assessed using H&E staining, lactate dehydrogenase (LDH) detection, ELISA, immunohistochemical staining, and WB. Furthermore, angiogenesis markers and associated proteins were detected using immunohistochemical staining and WB. RESULTS: Mice treated with STS exhibited significant improvements in cardiac function, smaller scar size, and low expression levels of α-smooth muscle actin and collagen I and III at 28 days following surgery, compared with the NS-treated group. Moreover, treatment with STS reduced eosinophil necrosis, the infiltration of inflammatory cells, plasma levels of LDH, high mobility group protein B1, interleukin-1β and tumor necrosis factor- α, and protein expression of these cytokines at 3 days. Macrophage infiltration was also decreased in the STS group in the early phase. Additionally, CD31+ vascular density, protein levels of hypoxia-inducible factor- 1α, and vascular endothelial growth factor were elevated in the STS-treated mice at 28 days. CONCLUSION: STS improved pathological remodeling post-MI, and the associated therapeutic effects may be a result of a decrease in myocardial necrosis, modulation of inflammation, and an increase in angiogenesis.
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Authors | Baoli Zhang, Peng Yu, Enyong Su, Jianguo Jia, Chunyu Zhang, Shiyao Xie, Zhenhui Huang, Ying Dong, Jinguo Ding, Yunzeng Zou, Hong Jiang, Junbo Ge |
Journal | Current pharmaceutical design
(Curr Pharm Des)
Vol. 28
Issue 9
Pg. 751-759
( 2022)
ISSN: 1873-4286 [Electronic] United Arab Emirates |
PMID | 34951571
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | Copyright© Bentham Science Publishers; For any queries, please email at [email protected]. |
Chemical References |
- Phenanthrenes
- Vascular Endothelial Growth Factor A
- tanshinone II A sodium sulfonate
|
Topics |
- Animals
- Cicatrix
(pathology)
- Disease Models, Animal
- Humans
- Inflammation
(metabolism)
- Mice
- Myocardial Infarction
(drug therapy)
- Myocardium
(metabolism)
- Neovascularization, Pathologic
(metabolism)
- Phenanthrenes
- Vascular Endothelial Growth Factor A
(metabolism)
- Ventricular Remodeling
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