Daidzin, 4', 7-dihydroxyisoflavone is an isoflavonic
phytoestrogen present in leguminous plants.
Traditional Chinese medicine utilizes
daidzin to treat various diseases such
diarrhea,
fever,
hepatitis, cardiac problems etc. In current study we examined the anticancer activity of
daidzin against human
cervical cancer in vitro. HeLa, human
cervical cancer cell line was purchased from ATCC and the cells were cultured with DMEM medium. The cytotoxic effect of
daidzin against HeLa cell line was analyzed with MTT assay. The IC-50 value was obtained at 20 µM hence the cells were treated with 20 µM of
daidzin for further analysis. ROS generation was assessed with
DCFH-DA staining and the induction of apoptosis was examined with Rhoadmine-123 staining.
Acridine orange and
ethidium bromide staining was done to examine the apoptotic and viable cells. Further the
matrigel cell adhesion assay was done to analyze the inhibitory property of
daidzin against
cancer cell adhesion. Apoptotic induction of
daidzin was examined by estimating the levels of
Caspase 8 & 9 using ELISA technique. Inflammatory and cell proliferation signaling
proteins were analyzed with qPCR analysis to confirm the anticancer activity of
daidzin against human
cervical cancer HeLa cell line.
Daidzin significantly generated ROS and altered the mitochondrial membrane permeability in HeLa cell line. The results of AO/EtBr staining prove
daidzin induced apoptosis in HeLa cell line and it also inhibited the cell adhesion property of HeLa which is reported in our
matrigel cell adhesion assay. It also increased the
caspases 8 & 9 which are key regulators of apoptosis.
Daidzin significantly decreased the expression of inflammatory gene and cell proliferating signaling molecule. To, conclude our results confirm
daidzin effectively decreased
inflammation and induced apoptosis in human
cervical cancer HeLa cell line.