Gene set enrichment analysis and ingenuity pathway analysis to identify biomarkers in Sheng-ji Hua-yu formula treated diabetic ulcers.

Abstract

ETHNOPHARMACOLOGICAL RELEVANCE:

Sheng-ji Hua-yu (SJHY) formula is a Chinese herbal prescription for diabetic ulcers (DUs) treatment, which can accelerate wound reconstruction and shorten the healing time. However, its mechanism role maintains unclear.

AIM OF THE STUDY:

To elucidate the molecular mechanisms of SJHY application on DUs.

MATERIALS AND METHODS:

To begin with, transcriptome sequencing was adopted to identified differentially expression mRNAs among normal ulcers, DUs, and DUs + SJHY treatment in vivo. Liquid chromatography-tandem mass spectrometry was applied for the quality control of SJHY formula. GO and KEGG enrichment analysis were used to identify the mechanisms underlying the therapeutic effect of SJHY formula, and then gene set enrichment analysis and ingenuity pathway analysis were conducted for functional analysis. Further, qPCR detection was performed in vivo for validation.

RESULTS:

SJHY administration could regulate the glucose metabolic process, AMPK and HIF-1 pathway to accelerate healing processes of DUs. Besides, CRHR1, SHH, and GAL were identified as the critical targets, and SLC6A3, GRP, FGF23, and CYP27B1 were considered as the upstream genes of SJHY treatment. Combined with animal experiments, the prediction results were validated in DUs mice model.

CONCLUSIONS:

This study used modular pharmacology analysis to identify the biomarkers of SJHY formula and provide the potential therapeutic targets for DUs treatment as well.

• Authors: Yi Ru, Ying Zhang, Yan-Wei Xiang, Ying Luo, Yue Luo, Jing-Si Jiang, Jian-Kun Song, Xiao-Ya Fei, Dan Yang, Zhan Zhang, Hui-Ping Zhang, Tai-Yi Liu, Shuang-Yi Yin, Bin Li, Le Kuai
• Journal: Journal of ethnopharmacology (J Ethnopharmacol) Vol. 285 Pg. 114845 (Mar 01 2022) ISSN: 1872-7573 [Electronic] Ireland
• PMID: 34800645 (Publication Type: Journal Article)
• Copyright: Copyright © 2021 Elsevier B.V. All rights reserved.

Chemical References

• Drugs, Chinese Herbal
• RNA, Messenger
• Sheng-ji Hua-yu

Topics

• Animals
• Humans
• Mice
• Diabetes Complications
• Diabetes Mellitus, Experimental
• Drugs, Chinese Herbal (therapeutic use)
• RNA, Messenger (genetics, metabolism)
• Skin Ulcer (drug therapy, etiology)
• Wound Healing (drug effects)