LncRNA FGF14-AS2 represses growth of prostate carcinoma cells via modulating miR-96-5p/AJAP1 axis.

Abstract

OBJECTIVE:

This investigation devoted to lncRNA FGF14 antisense RNA 2 (FGF14-AS2) in prostate carcinoma progression.

METHODS:

The levels of lncRNA FGF14-AS2, miR-96-5p, and Adherens junction-associated protein-1 (AJAP1) in prostate carcinoma were tested by Western blot and qRT-PCR. How these two genes interacted was confirmed by RNA immunoprecipitation and dualluciferase gene methods. The effect of FGF14-AS2/miR-96-5p/AJAP1 axis in prostate carcinoma progression was determined by MTT, Transwell, and nude mice tumor model.

RESULTS:

FGF14-AS2 was a downregulated lncRNA in prostate carcinoma tissue and cells. FGF14-AS2 could restrain miR-96-5p expression while miR-96-5p hampered AJAP1. FGF14-AS2 could effectively decrease the biological behaviors of prostate carcinoma cells, while knock-down of FGF14-AS2 triggered opposite results. Moreover, miR-96-5p mimic presented a cancer promoter role in prostate carcinoma cells. AJAP1 expression level could affect levels of proteins related to epithelial-mesenchymal transition. In vivo experiment suggested that overexpressing FGF14-AS2 could reverse the promotion of silenced AJAP1 on prostate carcinoma cell metastasis, thus to inhibit tumor growth.

CONCLUSION:

lncRNA FGF14-AS2 was a downregulated lncRNA in prostate carcinoma and influenced cell proliferation and metastasis. The influence relied on modulating miR-96-5p and its target gene AJAP1.

• Authors: Rubing Li, Yingcong Chen, Jingwei Wu, Xiaobo Cui, Sinian Zheng, Huaqing Yan, Yiming Wu, Feng Wang
• Journal: Journal of clinical laboratory analysis (J Clin Lab Anal) Vol. 35 Issue 11 Pg. e24012 (Nov 2021) ISSN: 1098-2825 [Electronic] United States
• PMID: 34655124 (Publication Type: Journal Article)
• Copyright: © 2021 The Authors. Journal of Clinical Laboratory Analysis published by Wiley Periodicals LLC.

Chemical References

• AJAP1 protein, human
• Cell Adhesion Molecules
• MIRN96 microRNA, human
• MicroRNAs
• RNA, Long Noncoding

Topics

• Animals
• Cell Adhesion Molecules (genetics, metabolism)
• Disease Progression
• Humans
• Male
• Mice
• Mice, Nude
• MicroRNAs (genetics, metabolism)
• Prostate (metabolism)
• Prostatic Neoplasms (genetics, metabolism, pathology)
• RNA, Long Noncoding (genetics, metabolism)