Abstract |
Toxoplasmosis is a worldwide parasitosis that affects one-third of the population. People at risk, such as immunocompromised patients ( AIDS, chemotherapy treatment) or fetuses (maternal-fetal transmission) can develop severe forms of the disease. The antiparasitic activity of extracts of different polarities ( n-heptane, MeOH, MeOH/H2O) of 10 tree species endemic to temperate regions was investigated against Toxoplasma gondii infection in vitro. Our results showed that the n-heptane extract of the black alder (Alnus glutinosa) exhibited a significant antiparasitic activity without any cytotoxicity at the tested concentrations, with an IC50 of up to 25.08 μg/mL and a selectivity index higher than 3.99. The chemical profiling of this extract revealed triterpenes as major constituents. The ability of commercially available triterpene ( betulin, betulinic acid, and betulone) to inhibit the growth of T. gondii was evaluated and showed growth inhibition rates of 44%, 49%, and 99% at 10 μM, respectively.
|
Authors | Pierre Darme, Jérémy Spalenka, Jane Hubert, Sandie Escotte-Binet, Laurent Debelle, Isabelle Villena, Charlotte Sayagh, Nicolas Borie, Agathe Martinez, Benjamin Bertaux, Laurence Voutquenne-Nazabadioko, Jean-Hugues Renault, Dominique Aubert |
Journal | Antimicrobial agents and chemotherapy
(Antimicrob Agents Chemother)
Vol. 66
Issue 1
Pg. e0109821
(01 18 2022)
ISSN: 1098-6596 [Electronic] United States |
PMID | 34633849
(Publication Type: Journal Article)
|
Chemical References |
- Antiparasitic Agents
- Plant Extracts
- Triterpenes
|
Topics |
- Alnus
- Antiparasitic Agents
(pharmacology)
- Humans
- Plant Bark
- Plant Extracts
(pharmacology)
- Toxoplasma
- Triterpenes
(pharmacology)
|