A conservative distribution of tridomain NDP-heptose synthetases in actinobacteria.

Abstract

Heptoses are important structural components of Gram-negative bacterium cell wall and participate in bacterial colonization, infection, and immune recognition. Current knowledge of NDP-heptose originating from D-sedoheptulose 7-phosphate in Grampositive bacterium remains limited. Here, in silico analysis suggested that the special tridomain NDP-heptose synthetases with isomerase, kinase, and nucleotidyltransferase activities are conservatively distributed in Actinobacteria class of Gram-positive bacterium. Enzymatical characterization of the tridomain proteins from different strains showed that they are involved in ADP-D-glycero-β-D-manno-heptose biosynthesis despite the unexpected discovery of kinase activities deficient in some proteins. The presence of three types of NDP-heptose synthetases in Gram-positive bacterium suggests that it is also a rich source of heptoses and the heptose moieties may play important roles in vivo. Our work updates the understanding of NDP-heptose biosynthesis in Gram-positive bacterium and lays a solid foundation for further physiological function explorations.

• Authors: Yue Tang, Wei Tang, Min Wang, Zhilong Zhang, Yihua Chen
• Journal: Science China. Life sciences (Sci China Life Sci) Vol. 65 Issue 5 Pg. 1014-1023 (05 2022) ISSN: 1869-1889 [Electronic] China
• PMID: 34632535 (Publication Type: Journal Article)
• Copyright: © 2021. Science China Press and Springer-Verlag GmbH Germany, part of Springer Nature.

Chemical References

• Heptoses
• Ligases

Topics

• Actinobacteria (genetics, metabolism)
• Heptoses (chemistry, metabolism)
• Ligases