Abstract |
Esophageal squamous cell carcinoma (ESCC) is common cancer in China. At the same time, microRNA-196b (miR-196b) has different promotion/inhibition effects in different cancers. The study aims to reveal the role of miR-196b in ESCC and explore its prognostic value. The expression of miR-196b in ESCC samples and cell lines was detected to explore the expression pattern of miR-196b in ESCC. Kaplan-Meier method was conducted for survival rate and Multivariate Cox analysis was used to explore the clinical significance of miR-196b in ESCC. The Cell Counting Kit-8 (CCK-8) assay, transwell migration and invasion tests were used to determine the biological function of miR-196b in ESCC. The relationship of miR-196b and SOCS2 in ESCC was detected by luciferase activity assay and RIP assay. Both in ESCC tissues and cell lines, miR-196b expression was up-regulated. miR-196b expression is related to TNM stage and lymph node metastasis. Combining with the results of Multivariate Cox regression analysis, miR-196b may be a potential independent prognostic marker for ESCC patients. The results of the functional analysis showed that miR-196b inhibitor can reduce cell proliferation, migration and invasion in ESCC cells. Besides, the suppressor of cytokine signaling 2 (SOCS2) is the target of miR-196b in ESCC. miR-196b may exist as a tumor-promoting factor in ESCC and enhance the proliferation abilities, migration capacities, and invasion potential of ESCC cells by targeting SOCS2. miR-196b and SOCS2 have a close negative correlation in ESCC, which may be used as a clinically poor prognostic biomarker and therapeutic target for ESCC.
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Authors | Jinlong Xu, Jinmei Wang, Lili Liu, Lin Chen, Songliu Hu, Feng Liu |
Journal | Bioengineered
(Bioengineered)
Vol. 12
Issue 1
Pg. 7737-7746
(12 2021)
ISSN: 2165-5987 [Electronic] United States |
PMID | 34605350
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MIRN196 microRNA, human
- MicroRNAs
- SOCS2 protein, human
- Suppressor of Cytokine Signaling Proteins
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Topics |
- Aged
- Cell Line, Tumor
- Disease Progression
- Esophageal Neoplasms
(diagnosis, genetics, mortality, pathology)
- Esophageal Squamous Cell Carcinoma
(diagnosis, genetics, mortality, pathology)
- Esophagus
(pathology)
- Female
- Humans
- Male
- MicroRNAs
(genetics, metabolism)
- Middle Aged
- Prognosis
- Suppressor of Cytokine Signaling Proteins
(genetics, metabolism)
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