Abstract | OBJECTIVE: To elucidate the active compounds and the molecular mechanism of Cyathula Officinalis as a drug treatment for rheumatoid arthritis (RA). METHODS: The target genes of active ingredients from Cyathula Officinalis were obtained from bioinformatics analysis tool for the molecular mechanism of traditional Chinese medicine. The protein- protein interaction between the target genes were analyzed using STRING and Genemania. The transcriptome of RA patients compared to healthy people (GSE121894) were analyzed using R program package Limma. The relative expression of the target genes was obtained from the RNA-seq datasets. The molecular docking analyses were processed based on the molecular model of estrogen receptor 1 (ESR1) binding with estradiol (PDB ID:1A52). The binding details were analyzed by SYBYL. RESULTS:
Inokosterone, ecdysterone, and cyaterone were the 3 active ingredients from Cyathula Officinalis that bind to target genes. Of all the significantly changed genes from RA patients, ESR1, ADORA1, and ANXA1 were significantly increased in mRNA samples of RA patients. CONCLUSION:
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Authors | Ji-Hao Mo, Han-Kun Xie, Ye-Mian Zhou, Sihan-Benjamin Ng, Shao-Xia Li, Lei Wang |
Journal | Chinese journal of integrative medicine
(Chin J Integr Med)
Vol. 27
Issue 10
Pg. 767-773
(Oct 2021)
ISSN: 1672-0415 [Print] China |
PMID | 34432202
(Publication Type: Journal Article)
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Copyright | © 2021. The Chinese Journal of Integrated Traditional and Western Medicine Press and Springer-Verlag GmbH Germany, part of Springer Nature. |
Chemical References |
- Cholestenes
- Estrogen Receptor alpha
- Pharmaceutical Preparations
- inokosterone
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Topics |
- Arthritis, Rheumatoid
(drug therapy, genetics)
- Cholestenes
- Estrogen Receptor alpha
- Humans
- Molecular Docking Simulation
- Pharmaceutical Preparations
|