Abstract | BACKGROUND: METHODS: To investigate this hypothesis, combinations of the known GPX1 inhibitors 9-chloro-6-ethyl-6H-[1,2,3,4,5]pentathiepino[6,7-b] indole (CEPI) or mercaptosuccinic acid (MSA) with PDT induced by the photosensitizer (PS) temoporfin (5,10,15,20-tetra(m-hydroxyphenyl)chlorin, mTHPC) were studied in vitro. This new combinatory approach was intended to accumulate ROS formed during PDT via blockage of GPX1-catalyzed H2O2 degradation, and thus to enhance PDT-induced phototoxicity. Five human cancer cell lines from tumor origins treatable with PDT were utilized to investigate ROS generation, apoptosis induction, and cell cycle distribution. RESULTS: Synergy was identified with both GPX1 inhibitors, but not in all cell lines. ROS levels were increased after combined treatment with mTHPC and CEPI, but not MSA, in some cell lines, indicating that oxidative stress and ROS accumulation were enhanced by CEPI. Surprisingly, enhanced apoptosis induction was also observed with MSA afterwards, suggesting that other pathways contributed to the initiation of apoptosis. Cell cycle analysis confirmed apoptosis induction via the detection of DNA fragmentation. CONCLUSION: A combination of GPX1 inhibitors with mTHPC- PDT has the potential to generate synergistic effects and to increase overall phototoxicity, but the success of this combination approach was dependent on cancer type, and even antagonistic effects can occur.
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Authors | Carsten Lange, Patrick J Bednarski |
Journal | Photodiagnosis and photodynamic therapy
(Photodiagnosis Photodyn Ther)
Vol. 36
Pg. 102478
(Dec 2021)
ISSN: 1873-1597 [Electronic] Netherlands |
PMID | 34375776
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Elsevier B.V. All rights reserved. |
Chemical References |
- Mesoporphyrins
- Photosensitizing Agents
- Reactive Oxygen Species
- Hydrogen Peroxide
- Glutathione Peroxidase
- temoporfin
- Glutathione Peroxidase GPX1
- GPX1 protein, human
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Topics |
- Apoptosis
- Cell Line, Tumor
- Glutathione Peroxidase
- Humans
- Hydrogen Peroxide
(pharmacology)
- Mesoporphyrins
- Photochemotherapy
(methods)
- Photosensitizing Agents
(pharmacology, therapeutic use)
- Reactive Oxygen Species
- Glutathione Peroxidase GPX1
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