Objective: To analyze the
long non-coding RNA (
lncRNA) and
messenger RNA (
mRNA) co-expression network changes induced by mtDNA3010A/G mutation in acute
hypoxia, and to investigate the role of key
lncRNA and
mRNA in the regulation of gene expression induced by
hypoxia. Methods: The genotype combinations A-C-C and G-C-C of
mitochondrial DNA 3010-5178-10400 were screened, and genotypes of mtDNA3010A and mtDNA3010G fusion cells were constructed by using
osteosarcoma cell treated by
ethidium bromide without mitochondrion (ρ0206 cell) as donors.
After treatment with 1% O2 24 h, the
lncRNA -
mRNA expression chip was applied to detect the differently expressed
lncRNA and
mRNA in two kinds of fusing cells, and fluorescence quantitative polymerase chain method was used to verify differently expressed
mRNA. Bioinformatics methods were applied to build co-expression network of
lncRNA-
mRNA, predict target genes of differently expressed
lncRNA, and the functions of differently expressed
mRNA and target genes predicted by
lncRNA were also analyzed based on gene ontology (GO) and the Kyoto encyclopedia of genes and genomes (KEGG) forecast analysis. Results:
After treatment with 1% O2 for 24 h, compared with mtDNA3010G fusion cells: 688 lncRNAs were up-regulated, 21 were more than 2 times; 1098 were down-regulated, and 4 were more than 2 times. There were 1151
mRNA expressions up-regulated, 14 were more than 2 times, 539
mRNA expressions were down-regulated, and 3 were more than 2 times. Conclusion: MtDNA3010A/G genotype mutation under
hypoxia is able to affect the
lncRNA-
mRNA regulatory network, and the differentially expressed
lncRNA and
mRNA may play an important role in regulation network of gene expression induced by
hypoxia, which is expected to be a target for the regulation of
hypoxia reaction from the perspective of mitochondria.