Non-coding Natural Antisense Transcripts: Analysis and Application.

Abstract

Non-coding natural antisense transcripts (ncNATs) are regulatory RNA molecules that are overlapping with as well as complementary to other transcripts. These transcripts are implicated in a broad variety of biological and pathological processes, including tumorigenesis and oncogenic progression. With this complex field still in its infancy, annotations, expression profiling and functional characterisations of ncNATs are far less comprehensive than those for protein-coding genes, pointing out substantial gaps in the analysis and characterisation of these regulatory transcripts. In this review, we discuss ncNATs from an analysis perspective, in particular regarding the use of high-throughput sequencing strategies, such as RNA-sequencing, and summarize the unique challenges of investigating the antisense transcriptome. Finally, we elaborate on their potential as biomarkers and future targets for treatment, focusing on cancer.

• Authors: Julian C Krappinger, Lilli Bonstingl, Katrin Pansy, Katja Sallinger, Nick I Wreglesworth, Lukas Grinninger, Alexander Deutsch, Amin El-Heliebi, Thomas Kroneis, Ramsay J Mcfarlane, Christoph W Sensen, Julia Feichtinger
• Journal: Journal of biotechnology (J Biotechnol) Vol. 340 Pg. 75-101 (Nov 10 2021) ISSN: 1873-4863 [Electronic] Netherlands
• PMID: 34371054 (Publication Type: Journal Article, Review)
• Copyright: Copyright © 2021 The Authors. Published by Elsevier B.V. All rights reserved.

Chemical References

• RNA, Antisense

Topics

• Base Sequence
• High-Throughput Nucleotide Sequencing
• RNA, Antisense (genetics)
• Sequence Analysis, RNA
• Transcriptome (genetics)