Resveratrol has been shown to preserve organ function and improve survival in
hemorrhagic shock rat models. This study investigated whether seven days of oral
resveratrol could improve hemodynamic response to
hemorrhage and confer benefits on risk of
acute kidney injury (AKI) without inducing coagulopathy in a canine model. Twelve greyhound dogs were randomly allocated to receive oral
resveratrol (1000 mg/day) or placebo for seven days prior to inducing
hemorrhage until a targeted mean blood pressure of ≤40 mmHg was achieved. AKI
biomarkers and coagulation parameters were measured before, immediately following, and two hours after
hemorrhage. Dogs were euthanized, and renal tissues were examined at the end of the experiment. All investigators were blinded to the treatment allocation. A linear mixed model was used to assess effect of
resveratrol on AKI
biomarkers and coagulation parameters while adjusting for volume of blood loss. A significant larger volume of blood loss was required to achieve the
hypotension target in the
resveratrol group compared to placebo group (median 64 vs. 55 mL/kg respectively, p = 0.041). Although histological evidence of AKI was evident in all dogs, the renal tubular injury scores were not significantly different between the two groups, neither were the AKI
biomarkers. Baseline (pre-
hemorrhage) maximum clot firmness on the Rotational Thromboelastometry (ROTEM®) was stronger in the
resveratrol group than the placebo group (median 54 vs. 43 mm respectively, p = 0.009). In summary, seven days of oral
resveratrol did not appear to induce increased
bleeding risk and could improve greyhound dogs' blood pressure tolerance to severe
hemorrhage. Renal protective effect of
resveratrol was, however, not observed.