Abstract |
Intrauterine infection is one of the most important causes of maternal death. In perinatal emergency, we often miss an opportunity to obtain culture specimens. In this study, we tried to examine whether we investigated whether bacteria causing infection can be detected from a formalin-fixed paraffin-embedded (FFPE) placental specimen. We examined the placenta from a maternal invasive infection that resulted in infectious abortion at 18 weeks of gestation. The case was diagnosed by acute fever and abdominal pain, and the patient was cured after 3 weeks of intensive antimicrobial treatment. Four Streptococcus pyogenes strains were isolated from vaginal fluid and blood cultures of the patient. All of the strain types were emm1/ST28. We amplified the V1-V2 region of 16S rRNA from an FFPE placental specimen and sequencing was performed using a next-generation sequencer (NGS). Taxonomic analysis was then performed for sequenced data. We succeeded in detecting causative pathogens from the FFPE placenta: 69.1% of the predominantly identified bacteria were S. pyogenes and other small populations of bacteria were detected. Our results revealed the utility of NGS for 16S rRNA analysis of an FFPE placenta. This method may reveal previous perinatal invasive infections of unknown origin retrospectively.
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Authors | Tasuku Mariya, Toyotaka Sato, Yuya Fujibe, Manami Ishido, Hiroshi Shimada, Terufumi Kubo, Yoko Nagai, Wataru Arai, Suguru E Tanaka, Kyota Ashikawa, Yoshiyuki Sakuraba, Shinichi Ishioka, Shin-Ichi Yokota, Tsuyoshi Saito |
Journal | Medical molecular morphology
(Med Mol Morphol)
Vol. 54
Issue 4
Pg. 374-379
(Dec 2021)
ISSN: 1860-1499 [Electronic] Japan |
PMID | 34287707
(Publication Type: Case Reports, Journal Article)
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Copyright | © 2021. The Japanese Society for Clinical Molecular Morphology. |
Chemical References |
- RNA, Ribosomal, 16S
- Formaldehyde
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Topics |
- Female
- Formaldehyde
- High-Throughput Nucleotide Sequencing
- Humans
- Paraffin Embedding
- Placenta
- Pregnancy
- RNA, Ribosomal, 16S
(genetics)
- Retrospective Studies
- Streptococcus pyogenes
(genetics)
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