Oxidative stress plays a critical role in the development of cardiac remodeling and
heart failure.
Lutein, the predominant nonvitamin A
carotenoid, has been shown to have profound effects on oxidative stress. However, the effect of
lutein on
angiotensin II (Ang II)-induced cardiac remodeling and
heart failure remains unknown.
OBJECTIVE: In vitro experiments with isolated neonatal rat cardiomyocytes (NRCMs) and cardiac fibroblasts (CFs) revealed that
lutein significantly attenuated Ang II-induced
collagen expression in CFs, and cardiomyocyte
hypertrophy. The Ang II-induced increases in
superoxide generation,
inflammation and apoptosis in cultured CFs were strikingly prevented by
lutein. In vivo,
fibrosis, hypertrophic cardiomyocyte and
superoxide generation were analyzed, and
lutein was demonstrated to confer resistance to Ang II-induced cardiac remodeling in mice. Mechanistically,
RNA sequencing revealed that
interleukin-11 (IL-11) expression was significantly upregulated in mouse hearts in response to Ang II infusion and was significantly suppressed in the hearts of
lutein-treated mice. Furthermore,
IL-11 overexpression blocked the effects of
lutein on
fibrosis and oxidative stress in CFs and impaired the protective effect of
lutein on cardiac remodeling. Notably, we discovered that
lutein could reduce Ang II-induced
IL-11 expression, at least partly through the regulation of activator
protein (AP)-1 expression and activity.
CONCLUSIONS: