It is an urgent need to tackle drug-resistance microbial
infections that are associated with implantable biomedical devices.
Host defense peptide-mimicking
polymers have been actively explored in recent years to fight against
drug-resistant microbes. Our recent report on
lithium hexamethyldisilazide-initiated superfast polymerization on
amino acid N-carboxyanhydrides enables the quick synthesis of
host defense peptide-mimicking
peptide polymers. Here we reported a facile and cost-effective thermoplastic
polyurethane (TPU) surface modification of
peptide polymer (DLL: BLG = 90 : 10) using plasma surface activation and substitution reaction between
thiol and
bromide groups. The
peptide polymer-modified TPU surfaces exhibited board-spectrum antibacterial property as well as effective contact-killing ability in vitro. Furthermore, the
peptide polymer-modified TPU surfaces showed excellent biocompatibility, displaying no
hemolysis and cytotoxicity. In vivo study using methicillin-resistant Staphylococcus aureus (MRSA) for subcutaneous implantation infectious model showed that
peptide polymer-modified TPU surfaces revealed obvious suppression of
infection and great histocompatibility, compared to bare TPU surfaces. We further explored the antimicrobial mechanism of the
peptide polymer-modified TPU surfaces, which revealed a surface contact-killing mechanism by disrupting the bacterial membrane. These results demonstrated great potential of the
peptide-modified TPU surfaces for practical application to combat
bacterial infections that are associated with implantable materials and devices.