G3BP1 may serve as a potential biomarker of proliferation, apoptosis, and prognosis in oral squamous cell carcinoma.

Abstract

BACKGROUND:

G3BP1 is a prognostic biomarker for many types of cancers; however, its role in oral squamous cell carcinoma remains unclear. We investigated the role of G3BP1 as a potential biomarker for proliferation, apoptosis, and prognosis in oral squamous cell carcinoma.

METHODS:

We obtained samples of normal oral mucosa (n = 17), oral squamous cell carcinoma tissues (n = 61), and paired adjacent tissues (n = 47) from Xiangya Hospital for immunohistochemical evaluation to measure the expression of G3BP1, E-cadherin, Ki67, and Cleaved-caspase3. Using data from The Cancer Genome Atlas, we performed bioinformatics analysis to investigate the prognosis, functions, signaling pathways, and immune infiltrate significance related to G3BP1 in oral squamous cell carcinoma.

RESULTS:

The G3BP1 protein level was significantly upregulated in oral squamous cell carcinoma tissues and was also positively associated with Ki67 and negatively associated with Cleaved-caspase3. Based on information available in online database, the G3BP1 mRNA level was significantly higher in oral squamous cell carcinoma than in normal tissues. High G3BP1 mRNA levels were associated with poor overall survival rates in patients with oral squamous cell carcinoma. Enrichment analysis showed that G3BP1 was involved in the helicase/catalytic/ATPase activity functions and spliceosome/RNA transport/ cell cycle pathways. Furthermore, G3BP1 mRNA levels were positively associated with CD4+ T-cell infiltration.

CONCLUSIONS:

G3BP1 may serve as a potential biomarker for proliferation, apoptosis, and prognosis of oral squamous cell carcinoma.

• Authors: Xin Hu, Kun Xia, Haofeng Xiong, Tong Su
• Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 50 Issue 10 Pg. 995-1004 (Nov 2021) ISSN: 1600-0714 [Electronic] Denmark
• PMID: 33987877 (Publication Type: Journal Article)
• Copyright: © 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.

Chemical References

• Biomarkers
• Poly-ADP-Ribose Binding Proteins
• RNA Recognition Motif Proteins
• DNA Helicases
• G3BP1 protein, human
• RNA Helicases

Topics

• Apoptosis
• Biomarkers
• Carcinoma, Squamous Cell (genetics)
• Cell Proliferation
• DNA Helicases (genetics, metabolism)
• Gene Expression Regulation, Neoplastic
• Head and Neck Neoplasms
• Humans
• Mouth Neoplasms (genetics)
• Poly-ADP-Ribose Binding Proteins (genetics, metabolism)
• Prognosis
• RNA Helicases (genetics, metabolism)
• RNA Recognition Motif Proteins (genetics, metabolism)
• Squamous Cell Carcinoma of Head and Neck