Abstract |
Clostridium perfringens beta2 (CPB2) toxin can cause intestinal damage and inflammatory responses in a variety of animals, which seriously endanger the healthy development of animal husbandry. Increasing evidence has demonstrated that microRNAs ( miRNAs) can play an important regulatory role in the process of pathogenic infection. In our previous study, we found that miR-204 was highly expressed in the ileum tissues of the susceptible group diarrhea piglets after infection with Clostridium perfringens (C. perfringens) type C. In this study, we found that miR-204 was also up-regulated in different time points after CPB2 toxin treatment. Overexpression of miR-204 promoted apoptosis and inflammatory response of intestinal porcine epithelial cells (IPEC-J2), whereas the opposite results were displayed after transfected with miR-204 inhibitor. Furthermore, the luciferase reporter assays confirmed that BCL2L2 was a direct target gene of miR-204. Interestingly, we found that overexpression BCL2L2 attenuated the apoptosis and inflammatory response of CPB2 toxin induced IPEC-J2 cells. In conclusion, these results find that miR-204 promotes the apoptosis and intensify inflammatory response of CPB2 toxin induced IPEC-J2 cells via targeting BCL2L2. These data provide a valuable reference for the piglets resistance diarrhea at the molecular level.
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Authors | Wei Wang, Qiaoli Yang, Xiaoyu Huang, Ruirui Luo, Kaihui Xie, Xiaoli Gao, Zunqiang Yan, Pengfei Wang, Juanli Zhang, Jiaojiao Yang, Bo Zhang, Shuangbao Gun |
Journal | Microbial pathogenesis
(Microb Pathog)
Vol. 156
Pg. 104906
(Jul 2021)
ISSN: 1096-1208 [Electronic] England |
PMID | 33965507
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Elsevier Ltd. All rights reserved. |
Chemical References |
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Topics |
- Animals
- Apoptosis
- Clostridium perfringens
(genetics)
- Diarrhea
- Epithelial Cells
- MicroRNAs
(genetics)
- Swine
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