Abstract | BACKGROUND: METHODS: Differentially expressed miRNAs and target genes related to liver cancer were predicted by bioinformatics analyses, and their expression was determined in liver tissues of patients with liver cancer and liver cancer cells. The RNA immunoprecipitation (RIP), RNA pull-down and dual luciferase reporter assay were used to examine the binding affinity among XIST and miR-424-5p and OGT. Then, gain- and loss-of-function assays were conducted to evaluate the effects of the XIST/miR-424-5p/OGT axis on malignant phenotypes. A nude mouse model of liver cancer was further established for in vivo substantiation. RESULTS: XIST and OGT were up-regulated in liver cancer tissues and cells, responsible for poor prognosis in patients with liver cancer, while miR-424-5p was down-regulated. XIST competitively bound to miR-424-5p to increase OGT expression. XIST silencing inhibited malignant phenotypes of liver cancer cells, while miR-424-5p down-regulation negated its effect. miR-424-5p suppressed RAF1 glycosylation by negatively regulating OGT expression and promoted its ubiquitination/degradation. Furthermore, XIST knockdown inhibited tumour growth and metastasis in nude mice, while ectopic OGT reversed its effect. CONCLUSION:
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Authors | Deng Ning, Jin Chen, Pengcheng Du, Qiumeng Liu, Qi Cheng, Xue Li, Bixiang Zhang, Xiaoping Chen, Li Jiang |
Journal | Liver international : official journal of the International Association for the Study of the Liver
(Liver Int)
Vol. 41
Issue 8
Pg. 1933-1944
(08 2021)
ISSN: 1478-3231 [Electronic] United States |
PMID | 33909326
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. |
Chemical References |
- MIRN424 microrna, human
- MicroRNAs
- RNA, Long Noncoding
- N-Acetylglucosaminyltransferases
- O-GlcNAc transferase
- Proto-Oncogene Proteins c-raf
- Raf1 protein, human
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Topics |
- Animals
- Cell Proliferation
- Glycosylation
- Humans
- Liver Neoplasms
(genetics)
- Mice
- Mice, Nude
- MicroRNAs
(genetics)
- N-Acetylglucosaminyltransferases
(genetics)
- Proto-Oncogene Proteins c-raf
- RNA, Long Noncoding
(genetics)
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