Abstract |
Entamoeba histolytica is an intestinal parasite that causes dysentery and amebic liver abscess. E. histolytica has the capability to invade host tissue by union of virulence factor Gal/GalNAc lectin; this molecule induces an adherence-inhibitory antibody response as well as to protect against amebic liver abscess (ALA). The present work showed the effect of the immunization with PEΔIII-LC3-KDEL3 recombinant protein. In vitro, this candidate vaccine inhibited adherence of E. histolytica trophozoites to HepG2 cell monolayer, avoiding the cytolysis, and in a hamster model, we observed a vaccine-induced protection against the damage to tissue liver and the inhibition of uncontrolled inflammation. PEΔIII-LC3-KDEL3 reduced the expression of TNF-α, IL-1β, and NF-κB in all immunized groups at 4- and 7-day postinfection. The levels of IL-10, FOXP3, and IFN-γ were elevated at 7 days. The immunohistochemistry assay confirmed this result, revealing an elevated quantity of +IFN-γ cells in the liver tissue. ALA formation in hamsters immunized was minimal, and few trophozoites were identified. Hence, immunization with PEΔIII-LC3-KDEL3 herein prevented invasive amebiasis, avoided an acute proinflammatory response, and activated a protective response within a short time. Finally, this recombinant protein induced an increase of serum IgG.
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Authors | Sandra L Martínez-Hernández, Viridiana M Becerra-González, Martín H Muñoz-Ortega, Víctor M Loera-Muro, Manuel E Ávila-Blanco, Marina N Medina-Rosales, Javier Ventura-Juárez |
Journal | Journal of immunology research
(J Immunol Res)
Vol. 2021
Pg. 6697900
( 2021)
ISSN: 2314-7156 [Electronic] Egypt |
PMID | 33824880
(Publication Type: Journal Article)
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Copyright | Copyright © 2021 Sandra L. Martínez-Hernández et al. |
Chemical References |
- Antibodies, Protozoan
- Lectins
- Protozoan Proteins
- Protozoan Vaccines
- Recombinant Fusion Proteins
- Vaccines, Synthetic
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Topics |
- Animals
- Antibodies, Protozoan
(blood)
- Disease Models, Animal
- Entamoeba histolytica
(genetics, immunology)
- Humans
- Immunogenicity, Vaccine
- Lectins
(genetics, immunology)
- Liver
(immunology, parasitology, pathology)
- Liver Abscess, Amebic
(blood, parasitology, pathology, prevention & control)
- Male
- Mesocricetus
- Protozoan Proteins
(administration & dosage, genetics, immunology)
- Protozoan Vaccines
(administration & dosage, genetics, immunology)
- Recombinant Fusion Proteins
(administration & dosage, genetics, immunology)
- Vaccines, Synthetic
(administration & dosage, genetics, immunology)
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