Abstract | AIMS: METHODS: We analyzed the urethras of three groups of 18 female rats 30 days posttrauma by VD: control (no interventions); trauma (animals that had VD); and electrotherapy group (those that had VD and were treated with electrical stimulation). We compared the expression of vascular endothelial growth factor ( VEGF), nerve growth factor ( NGF), collagen types I and III (COL1a1 and COL3a1), and lysyl-oxidase like 1 (LOXL1) among the groups. Real-time reverse transcription-polymerase chain reaction, Western blot, and immunohistochemistry were used for molecule quantification. We used the Kruskal-Wallis test and analysis of variance for statistical analyses with p < 0.05 for significance. RESULTS: The COL1a1 gene expression was higher in the electrotherapy group than the trauma group (p = 0.036). COL3a1, VEGF, NGF, LOXL1 messenger RNA ( mRNA) expression did not differ among the groups (p ≥ 0.05). COL1a1, COL3a1, VEGF, NGF, LOXL1 protein levels did not significantly differ among the groups (p ≥ 0.05) in Western blot analysis or immunohistochemistry assays. CONCLUSIONS:
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Authors | Suellen M Feitosa, Maria A T Bortolini, Gisela R F Salerno, Andreisa P M Bilhar, Silvia S M Ihara, Manuel J Simões, Rodrigo A Castro |
Journal | Neurourology and urodynamics
(Neurourol Urodyn)
Vol. 40
Issue 4
Pg. 968-977
(04 2021)
ISSN: 1520-6777 [Electronic] United States |
PMID | 33769595
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Copyright | © 2021 Wiley Periodicals LLC. |
Chemical References |
- Vascular Endothelial Growth Factor A
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Topics |
- Animals
- Electric Stimulation Therapy
- Extracellular Matrix
- Female
- Rats
- Urethra
- Vagina
- Vascular Endothelial Growth Factor A
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