Glucosyl Hesperidin Has an Anti-diabetic Effect in High-Fat Diet-Induced Obese Mice.

Abstract

Glucosyl hesperidin (GH) is a water-soluble derivative of hesperidin, a citrus flavonoid. GH has various pharmacological effects, such as hypolipidemic and hypouricemic effects, and may therefore be a useful supplement or drug. In the present study, we evaluated the effects of long- and short-term intake of GH on hyperglycemia and macrophage infiltration into the adipose tissue of high-fat diet (HFD)-fed mice. Long-term (11-week) consumption of GH tended to reduce body weight and the fasting blood glucose concentration of the HFD-fed mice, and ameliorated glucose intolerance and insulin resistance, according to glucose and insulin tolerance tests. Additionally, although GH did not affect fat pad weight, it reduced HFD-induced macrophage infiltration into adipose tissue. Short-term (2-week) consumption of GH did not affect the HFD-induced increases in body weight or fasting blood glucose, and it did not ameliorate glucose intolerance or insulin resistance. However, short-term intake did reduce the HFD-induced macrophage infiltration and monocyte chemotactic protein 1 (MCP-1) expression in adipose tissue. Furthermore, hesperetin, which is an aglycone of GH, inhibited MCP-1 expression in 3T3-L1 adipocytes, 3T3-L1 adipocytes co-cultured with RAW264 macrophages, and tumor necrosis factor-α-treated 3T3-L1 adipocytes. The present findings suggest that daily consumption of GH may have preventive and/or therapeutic effects on obesity-related diseases, such as diabetes mellitus.

• Authors: Hiroki Yoshida, Rika Tsuhako, Chihiro Sugita, Masahiko Kurokawa
• Journal: Biological & pharmaceutical bulletin (Biol Pharm Bull) Vol. 44 Issue 3 Pg. 422-430 ( 2021) ISSN: 1347-5215 [Electronic] Japan
• PMID: 33642550 (Publication Type: Journal Article)

Chemical References

• Ccl2 protein, mouse
• Chemokine CCL2
• Glucosides
• Hypoglycemic Agents
• glucosyl hesperidin
• Hesperidin

Topics

• 3T3-L1 Cells
• Adipose Tissue (drug effects, immunology)
• Animals
• Chemokine CCL2 (genetics, immunology)
• Coculture Techniques
• Diet, High-Fat
• Disease Models, Animal
• Glucosides (pharmacology, therapeutic use)
• Hesperidin (analogs & derivatives, pharmacology, therapeutic use)
• Hyperglycemia (drug therapy, immunology)
• Hypoglycemic Agents (pharmacology, therapeutic use)
• Macrophages (drug effects, immunology)
• Male
• Mice
• Mice, Inbred C57BL
• Obesity (drug therapy, immunology)
• RAW 264.7 Cells