Cellular Trafficking of Glutathione Transferase M2-2 Between U373MG and SHSY-S7 Cells is Mediated by Exosomes.

Abstract

The enzyme glutathione transferase M2-2, expressed in human astrocytes, increases its expression in the presence of aminochrome and catalyzes the conjugation of aminochrome, preventing its toxic effects. Secretion of the enzyme glutathione transferase M2-2 from U373MG cells, used as a cellular model for astrocytes, has been reported, and the enzyme is taken up by neuroblastoma SYSH-S7 cells and provide protection against aminochrome. The present study provides evidence that glutathione transferase M2-2 is released in exosomes from U373MG cells, thereby providing a means for intercellular transport of the enzyme. With particular relevance to Parkinson disease and other degenerative conditions, we propose a new mechanism by which astrocytes may protect dopaminergic neurons against the endogenous neurotoxin aminochrome.

• Authors: Raúl Valdes, Alicia Armijo, Patricia Muñoz, Kjell Hultenby, Andres Hagg, Jose Inzunza, Ivan Nalvarte, Mukesh Varshney, Bengt Mannervik, Juan Segura-Aguilar
• Journal: Neurotoxicity research (Neurotox Res) Vol. 39 Issue 2 Pg. 182-190 (Apr 2021) ISSN: 1476-3524 [Electronic] United States
• PMID: 33555546 (Publication Type: Journal Article)

Chemical References

• Glutathione Transferase
• glutathione S-transferase Mu 2

Topics

• Astrocytes (metabolism)
• Cell Line, Tumor
• Dopaminergic Neurons (metabolism)
• Exosomes (metabolism)
• Glutathione Transferase (metabolism)
• Humans
• Protein Transport