During the occurring of cutaneous
trauma, increasing oxidative stress response in
wound site retards the progress of proliferation phase, impeding sequent efficient
wound repair. At the same time, high-quality healing also requires adequate new blood vessels in order to furnish the
wound site with a nutrient and
oxygen-sufficient environment. Here we synthesized a novel
hyaluronic acid (HA) material modified with a peroxidation inhibitor 2,2,6,6-tetramethylpiperidinyloxy (ATEMPO) for prevention of excessive
reactive oxygen species (ROS) and promotion of angiogenesis after full-thickness skin excision in rats.
Amines in ATEMPO attaching with carbonyls in HA chains was fabricated through N-acylation. The HA-g-
TEMPO exerted a ROS-scavenging and angiogenesis-promoting function in vitro. In acute
wound rat model, the
wound closure efficacy was significantly improved to almost 55% at day 6 in comparison to 49% of HA, and
wound sites in initial
wound phase was also narrowed down sharply. Moreover, initially formed blood vessels were found in
wound sites, further proved the angiogenesis-promoting function of HA-g-
TEMPO. More interestingly,
wound sites demonstrated an exciting regenerative healing effect which was characterized by marked skin appendages as well as reduced
scarring. Therefore, this strategy showed a promising future that could be considered as a reliable and effective method to cutaneous wound healing.