Abstract | BACKGROUND: METHODS: We conducted lncRNA and mRNA expression in lung adenocarcinoma and its SM tissue using microarray analysis. Quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR) revealed 10 differentially expressed lncRNAs. Gene ontology and pathway analysis were performed to test the gene effect. Possible target genes of lncRNAs were predicted based on precise algorithms. RESULTS: Microarray analysis found many significantly differentially expressed lncRNAs and mRNAs in lung adenocarcinoma compared with SM. qRT-PCR results aligned with those of the microarray analysis. The expression level of 10 lncRNAs showed the same trend (P<0.05). Biologic pathways known to be involved in cancer were identified among the differentially expressed mRNAs; these include cell adhesion molecules (related to 42 genes), focal adhesion (related to 31 genes), cytokine- cytokine receptor interaction (related to 48 genes), and extracellular matrix-receptor interaction (related to 23 genes). About 9,458 lncRNAs were found to have cis- or trans-genes. A total of 2,317 cis target genes were discovered to be abnormally expressed and could be regulated by lncRNAs in SM of lung adenocarcinoma. CONCLUSIONS:
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Authors | Houlei Wang, Annan Hu, Yun Liang, Ketao Wang, Xiaogang Zhou, Jian Dong |
Journal | Annals of translational medicine
(Ann Transl Med)
Vol. 8
Issue 22
Pg. 1516
(Nov 2020)
ISSN: 2305-5839 [Print] China |
PMID | 33313261
(Publication Type: Journal Article)
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Copyright | 2020 Annals of Translational Medicine. All rights reserved. |